Comparison of reverse transcription polymerase chain reaction, enzyme linked immunosorbent assay and virus isolation for the routine diagnosis of foot-and-mouth disease
Comparison of reverse transcription polymerase chain reaction, enzyme linked immunosorbent assay and virus isolation for the routine diagnosis of foot-and-mouth disease
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DOI：
10.1016/s0166-0934(97)00181-x
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发表时间：
1998-02-01
影响因子：
3.1
通讯作者：
Ferris, NP
中科院分区：
文献类型：
--
作者：
Reid, SM;Forsyth, MA;Ferris, NP
采用逆转录聚合酶链反应（RT-PCR）法与细胞培养病毒分离法和抗原检测ELISA法对166份野外临床标本进行口蹄疫初步诊断。病毒分离/ELISA检测阳性80份，RT-PCR检测阳性78份。RT-PCR在86份病毒分离/ELISA鉴定为阴性的样品中检测到口蹄疫病毒RNA，但在后者鉴定为阳性的13份样品中检测不到。这种RT-PCR不具有血清型特异性，因此cDNA产物仅表明存在口蹄疫病毒RNA。确认cDNA产物的特异性和血清型鉴定需要核苷酸序列分析。RT-PCR的价值在于，它可以迅速促进对现场分离株的分子分析，从而提供有关疫情来源的重要流行病学信息。然而，这是一项复杂的技术，需要专门的设备、专业知识和精制试剂，并且必须与目前的口蹄疫诊断程序结合使用。(C) 1998 Elsevier Science B.V.版权所有
A reverse transcription polymerase chain reaction (RT-PCR) method was compared with virus isolation in cell culture and the antigen detection ELISA for the primary diagnosis of foot-and-mouth disease (FMD) on 166 clinical samples from the field. Eighty samples were positive by virus isolation/ELISA and 78 by RT-PCR. The RT-PCR detected FMD viral RNA in 11 of the 86 samples assessed as negative by virus isolation/ELISA but conversely failed to diagnose 13 samples identified as positive by the latter procedures. This RT-PCR is not serotype-specific so a cDNA product is indicative of the presence of FMD viral RNA only. Confirmation of the specificity of the cDNA product and the identification of the serotype requires nucleotide sequence analysis. The value of the RT-PCR is that it can rapidly facilitate the molecular analysis of field isolates and thus provide important epidemiological information regarding the source of outbreaks. However, it is a sophisticated technique requiring specialised equipment, expertise and refined reagents and has to be used in conjunction with current procedures for FMD diagnosis. (C) 1998 Elsevier Science B.V. All rights reserved.