Identification of ribosomal protein L9 as a novel regulator of proinflammatory damage-associated molecular pattern molecules
Identification of ribosomal protein L9 as a novel regulator of proinflammatory damage-associated molecular pattern molecules
复制标题
鉴定核糖体蛋白 L9 作为促炎损伤相关分子模式分子的新型调节剂
DOI：
10.1007/s11033-021-07096-0
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发表时间：
2022
影响因子：
2.8
通讯作者：
Mori Shuji
中科院分区：
文献类型：
--
作者：
Watanabe Masahiro;Toyomura Takao;Wake Hidenori;Nishinaka Takashi;Hatipoglu Omer Faruk;Takahashi Hideo;Nishibori Masahiro;Mori Shuji
背景我们以前报道过晚期糖基化终产物（AGEs）通过与损伤相关分子模式分子（DAMP）的直接相互作用增强高迁移率族蛋白1（HMGB 1）的促炎活性。这表明，AGEs激活其他DAMPs，并导致我们寻找新的DAMPs能够与AGEs.Methods和resultsThe层析分析使用AGE-固定化凝胶揭示了核糖体蛋白家族是一个因素与AGEs的结合活性。核糖体蛋白L9（RPL 9）是核糖体蛋白家族的一员，在破裂细胞的离心上清液和脂多糖（LPS）刺激的脓毒症模型小鼠的血清中被发现，表现出与HMGB 1相似的特征性质。虽然HMGB 1增强LPS刺激的巨噬细胞样RAW264.7细胞中TNF-α的表达，但RPL 9几乎没有表现出这种活性。值得注意的是，RPL 9显著抑制HMGB 1加LPS刺激增强的TNF-α mRNA表达和蛋白质产生，表明其在DAMP诱导的促炎活性中的调节作用。差示扫描荧光分析结果表明，RPL 9与HMGB 1之间的直接相互作用可能是RPL 9抑制炎症反应的重要机制之一。结论RPL 9是一种新型的DAMP，在炎症反应中具有重要的调节作用。
BackgroundWe previously reported that advanced glycation endproducts (AGEs) increase the proinflammatory activity of high mobility group box-1 (HMGB1), a representative damage-associated molecular pattern molecule (DAMP), through their direct interaction. This suggested that AGEs activate other DAMPs and led us to search for novel DAMPs capable of interacting with AGEs.Methods and resultsThe chromatographic analysis using AGE-immobilized gel revealed the ribosomal protein family to be a factor with binding activity to AGEs. Ribosomal protein L9 (RPL9), a member of the ribosomal protein family, was found in the centrifugal supernatant of ruptured cells and in the serum of lipopolysaccharide (LPS)-stimulated sepsis model mice, exhibiting similar characteristic properties to HMGB1. Although HMGB1 potentiated LPS-stimulated TNF-α expression in macrophage-like RAW264.7 cells, RPL9 hardly exhibited this activity. Of note, RPL9 significantly suppressed the potentiated mRNA expression and protein production of TNF-α by HMGB1 plus LPS stimulation, suggesting its regulatory roles in DAMP-induced proinflammatory activity. Based on the differential scanning fluorimetric analysis, the direct interaction between RPL9 and HMGB1 may play a role in the suppressive effects of RPL9.ConclusionsThis study suggested that RPL9 is a novel type of DAMP with a regulatory role in the proinflammatory response and provided insight into the pathophysiology of inflammatory diseases.
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晚期糖基化终末产物减弱肿瘤坏死因子样弱凋亡诱导剂调节炎症反应的功能
影响因子：
4.3
作者：
Watanabe M;Toyomura T;Wake H;Liu K;Teshigawara K;Takahashi H;Nishibori M;Mori S
通讯作者：
Mori S
DOI：
10.1016/j.str.2009.09.015
发表时间：
2009-12-09
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Taylor DJ;Devkota B;Huang AD;Topf M;Narayanan E;Sali A;Harvey SC;Frank J
通讯作者：
Frank J
DOI：
10.1073/pnas.1602023113
发表时间：
2016-04-05
影响因子：
11.1
作者：
Hangai, Sho;Ao, Tomoka;Yanai, Hideyuki
通讯作者：
Yanai, Hideyuki
DOI：
10.3791/51809
发表时间：
2014-09-13
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Vivoli M;Novak HR;Littlechild JA;Harmer NJ
通讯作者：
Harmer NJ