Effect of glucose or glycerol as the sole carbon source on gene expression from the Salmonella prpBCDE promoter in Escherichia coli.
Effect of glucose or glycerol as the sole carbon source on gene expression from the Salmonella prpBCDE promoter in Escherichia coli.
复制标题
葡萄糖或甘油作为唯一碳源对大肠杆菌中沙门氏菌 prpBCDE 启动子基因表达的影响。
DOI：
10.1021/bp060193f
复制
发表时间：
2006
影响因子：
2.9
通讯作者：
Keasling,JayD
中科院分区：
文献类型：
--
作者：
Lee,SungKuk;Keasling,JayD
We have developed an expression system (Salmonella‐based pPro system) containing theSalmonella enterica prpBCDEpromoter (PprpB) andprpRencoding the positive transcriptional regulator of this promoter. In this study, the transcriptional efficiency of the pPro expression system was measured by placing the gene encoding the green fluorescent protein (gfp) under the control of PprpBand growing cells containing this construct in minimal medium supplemented with glucose or glycerol as a sole carbon source. In wild‐typeEscherichia coli(E. coli) BL21, the system exhibited high induced expression as well as high background expression; however, inE. coliJSB, asbm‐ygfDGHIdeletion mutant ofE. coliBL21(DE3), the system showed low background expression and high induced expression. The system exhibited homogeneous expression at the single‐cell level, highly regulatable expression over a wide range of propionate concentrations, and fully induced expression at a low propionate concentration relative to that needed to induce the system in rich, undefined medium. The expression system is comparable to the widely used T7 promoter‐driven expression systems in glucose or glycerol minimal medium.
