Immunological identification of the alternative oxidase of Acanthamoeba castellanii mitochondria
Immunological identification of the alternative oxidase of Acanthamoeba castellanii mitochondria
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DOI：
10.1016/s0014-5793(97)00676-5
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发表时间：
1997-07-07
期刊：
影响因子：
3.5
通讯作者：
Hryniewiecka, L
中科院分区：
文献类型：
--
作者：
Jarmuszkiewicz, W;Wagner, AM;Hryniewiecka, L
卡氏棘阿米巴原生动物的线粒体具有对氰化物不敏感的氧化酶，可与针对植物替代氧化酶的单克隆抗体发生交叉反应。免疫印迹揭示了三种单体形式（38、35 和 32 kDa）和极少量的单一 65 kDa 二聚体形式。交联研究表明，在植物中，替代氧化酶以二聚体的形式出现，而在阿米巴原虫中，它以单体的形式发挥作用。免疫学可检测的蛋白质水平随着阿米巴细胞培养时间的变化而变化。 35 kDa 蛋白质含量的增加伴随着抗氰呼吸活性的增加，(C) 1997 年欧洲生化协会联合会。
Mitochondria of the protozoa Acanthamoeba castellanii possess a cyanide-insensitive oxidase cross-reacting with monoclonal antibodies raised against the plant alternative oxidase. Immunoblotting revealed three monomeric forms (38, 35, and 32 kDa) and very low amounts of a single 65 kDa dimeric form. Cross-linking studies suggest that while in plants the alternative oxidase occurs as a dimer, in amoeba it functions as a monomer. Immunologically detectable protein levels change,vith the age of amoeba cell culture. Increased amounts of the 35 kDa protein are accompanied by an increase in the activity of cyanide-resistant respiration, (C) 1997 Federation of European Biochemical Societies.