Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain
Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain
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DOI：
10.1111/j.1365-2958.2008.06106.x
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Ragnini-Wilson, Antonella
中科院分区：
文献类型：
--
作者：
Casavola, Elena Caroli;Catucci, Alessandro;Ragnini-Wilson, Antonella
肌球蛋白V是一种基于肌动蛋白的运动，对各种细胞过程包括皮肤色素沉着、细胞分离和突触传递至关重要。肌球蛋白V通过其C-末端球状尾结构域（GTD）直接或间接地与货物结合受体结合来转运细胞器、囊泡和mRNA。我们已经使用芽殖酵母肌球蛋白V Myo 2 p阐明Myo 2 p如何与高尔基体后载体相互作用的机制。我们发现，Rab/Ypt蛋白Ypt 32 p，它与膜的trans-Golgi网络，分泌囊泡和内体，并与哺乳动物Rab 11，相互作用的Myo 2 p GTD内的一个区域先前确定为“囊泡结合区”。此外，我们发现，必需的肌球蛋白轻链1（Mlc 1 p），所需的囊泡交付在母芽颈部胞质分裂，结合到Myo 2 p GTD在一个区域重叠的Ypt 32 p。我们的数据是一致的Ypt 32 p和Mlc 1 p在调节后高尔基载体与Myo 2 p亚结构域II的相互作用的作用。
Myosin V is an actin-based motor essential for a variety of cellular processes including skin pigmentation, cell separation and synaptic transmission. Myosin V transports organelles, vesicles and mRNA by binding, directly or indirectly, to cargo-bound receptors via its C-terminal globular tail domain (GTD). We have used the budding yeast myosin V Myo2p to shed light on the mechanism of how Myo2p interacts with post-Golgi carriers. We show that the Rab/Ypt protein Ypt32p, which associates with membranes of the trans-Golgi network, secretory vesicles and endosomes and is related to the mammalian Rab11, interacts with the Myo2p GTD within a region previously identified as the 'vesicle binding region'. Furthermore, we show that the essential myosin light chain 1 (Mlc1p), required for vesicle delivery at the mother-bud neck during cytokinesis, binds to the Myo2p GTD in a region overlapping that of Ypt32p. Our data are consistent with a role of Ypt32p and Mlc1p in regulating the interaction of post-Golgi carriers with Myo2p subdomain II.