Trypanosoma caninum n. sp (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil
Trypanosoma caninum n. sp (Protozoa: Kinetoplastida) isolated from intact skin of a domestic dog (Canis familiaris) captured in Rio de Janeiro, Brazil
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DOI：
10.1017/s003118200900554x
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发表时间：
2009-04-01
期刊：
影响因子：
2.4
通讯作者：
Marzochi, M. C. A.
中科院分区：
文献类型：
--
作者：
Madeira, M. F.;Sousa, M. A.;Marzochi, M. C. A.
从巴西里约热内卢捕获的一只家犬的完整皮肤的无菌培养物中分离出一种未知的锥虫属，该家犬共感染了巴西利什曼原虫（Viannia）。在不同培养基中生长的培养物的吉姆萨染色涂片显示存在外鞭毛体、锥鞭毛体、球形鞭毛体、过渡阶段和分裂形式（外鞭毛体或球形鞭毛体）。在含有5%FCS的RPMI（15.2%）和DMEM（9.2%）培养基中观察到最高频率的锥鞭毛体，这些形式的平均长度分别为43.0和36.0 μ m。通过连续应用PCR分析的分子分析表明，该锥虫不同于克氏锥虫和锥虫。当应用特异性引物时，另一方面，使用引物D 75/D 76的靶向24 s α rDNA的D 7结构域的PCR策略在该分离物（储备A-27）中扩增了约250 bp的产物，与用T. cruzi和T. rangeli该菌不同于T. Cruzi主要是由于其锥鞭毛体形式和动质体的大小以及对巨噬细胞和锥蝽的感染性的缺乏。在形态上也不同于巴西报道的唾液锥虫。8个位点的同工酶分析表明，该菌的带型与T. rangeli和T.克鲁兹我们认为该分离物是一个新的锥虫种。名字T。犬建议。
An unknown Trypanosoma species was isolated from an axenic culture of intact skin from a domestic dog captured in Rio de Janeiro, Brazil, which was co-infected with Leishmania (Viannia) braziliensis. Giemsa-stained smears of cultures grown in different media revealed the presence of epimastigotes, trypomastigotes, spheromastigotes, transitional stages, and dividing forms (epimastigotes or spheromastigotes). The highest frequency of trypomastigotes was observed in RPMI (15.2%) and DMEM (9.2%) media containing 5% FCS, with a mean length of these forms of 43.0 and 36.0 mu m, respectively. Molecular analysis by sequential application of PCR assays indicated that this trypanosome differs from Trypanosoma cruzi and T. rangeli when specific primers were applied. On the other hand, a PCR strategy targeted to the D7 domain of 24s alpha rDNA, using primers D75/D76, amplified products of about 250 bp in that isolate (stock A-27), different from the amplification products obtained with T. cruzi and T. rangeli. This organism differs from T. cruzi mainly by the size of its trypomastigote forms and kinetoplasts and the absence of infectivity for macrophages and triatomine bugs. It is also morphologically distinct from salivarian trypanosomes reported in Brazil. Isoenzyme analysis at 8 loci demonstrated a very peculiar banding pattern clearly distinct from those of T. rangeli and T. cruzi. We conclude that this isolate is a new Trypanosoma species. The name T. caninum is suggested.