Autophagy and oxidative stress associated with gold nanoparticles
Autophagy and oxidative stress associated with gold nanoparticles
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DOI：
10.1016/j.biomaterials.2010.04.014
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发表时间：
2010-08-01
期刊：
影响因子：
14
通讯作者：
Yung, Lin-Yue L.
中科院分区：
文献类型：
--
作者：
Li, Jasmine J.;Hartono, Deny;Yung, Lin-Yue L.
已知元素金属纳米颗粒如镉和银会引起氧化应激，并且也具有高毒性。然而，对于金纳米颗粒（AuNPs），还没有很好地确定这些颗粒是否具有生物毒性。在这里，我们表明，金纳米粒子，这是由MRC-5人肺成纤维细胞在体外，诱导自噬伴随着氧化应激。我们还观察到自噬体的形成以及肺成纤维细胞中AuNP的摄取，以及处理样品中自噬蛋白、微管相关蛋白1轻链3（MAP-LC 3）和自噬基因7（ATG 7）的上调。AuNP处理的细胞还产生显著更多的脂质氢过氧化物（p值
Elemental metal nanoparticles like cadmium and silver are known to cause oxidative stress and are also highly toxic. Yet for gold nanoparticles (AuNPs), it is not well established whether these particles are biologically toxic. Here we show that AuNPs, which were taken up by MRC-5 human lung fibroblasts in vitro, induce autophagy concomitant with oxidative stress. We also observed formation of autophagosomes together with the uptake of AuNPs in the lung fibroblasts as well as upregulation of autophagy proteins, microtubule-associated protein 1 light chain 3 (MAP-LC3) and autophagy gene 7 (ATG 7) in treated samples. AuNP treated cells also generated significantly more lipid hydroperoxides (p-value