Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients
Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients
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DOI：
10.1016/s0015-0282(99)00442-2
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发表时间：
1999-12-01
影响因子：
6.7
通讯作者：
Oehninger, S
中科院分区：
文献类型：
--
作者：
Barroso, G;Barrionuevo, M;Oehninger, S
目的：检测单个卵泡中血管内皮生长因子（VEGF）、一氧化氮（NO）和瘦素水平，并研究其与卵泡周血流、卵泡代谢指标以及相应卵母细胞和胚胎发育潜力的关系。设计：前瞻性研究。设置：学术、三级医疗机构。患者：未选择的IVF患者。干预：卵泡周围血流的彩色脉冲多普勒分析;氧分压（pO（2））、二氧化碳分压（pCO（2））、pH和VEGF的测定。卵泡液中瘦素和NO水平。主要结果测量：受精和第3天胚胎形态和卵裂。结果：研究了来自16名患者的55个卵泡液样本。平均卵泡液水平如下：VEGF，1，046 +/- 863.7 pglmL（范围，
Objective(s): To measure vascular endothelial growth factor (VEGF), nitric oxide (NO) and leptin levels in individual ovarian follicles and to examine their relationships with perifollicular blood flow, follicular metabolic indices, and the developmental potential of the corresponding oocyte and embryo.Design: Prospective study.Setting: Academic, tertiary care institution.Patient(s): Unselected IVF patients.Intervention(s): Color-pulsed Doppler analysis of perifollicular blood flow; determination of partial pressure of oxygen (pO(2)), partial pressure of carbon dioxide (pCO(2)), and pH and VEGF. leptin and NO levels in follicular fluid.Main Outcome Measure(s): Fertilization and day 3 embryo morphology and cleavage.Result(s): Fifty-five follicular fluid samples from 16 patients were studied. Mean follicular fluid levels were as follows: VEGF, 1,046 +/- 863.7 pglmL (range,