SYNTHESIS AND SECRETION OF MATRIX-DEGRADING METALLOPROTEASES BY HUMAN SKELETAL-MUSCLE SATELLITE CELLS
SYNTHESIS AND SECRETION OF MATRIX-DEGRADING METALLOPROTEASES BY HUMAN SKELETAL-MUSCLE SATELLITE CELLS
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DOI：
10.1002/aja.1002020109
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发表时间：
1995-01-01
影响因子：
2.5
通讯作者：
HOLLAND, PC
中科院分区：
文献类型：
--
作者：
GUERIN, CW;HOLLAND, PC
用细胞培养上清液酶谱和Northern印迹杂交研究了人骨骼肌卫星细胞基质降解金属蛋白酶(MMPs)的表达。明胶底物凝胶中的酶谱分析表明，卫星细胞合成和分泌72 kDa明胶酶(MMP2)。此外，佛波酯处理卫星细胞培养物可诱导92 kDa明胶酶(MMP9)活性。在酪蛋白底物凝胶上，对照和佛波酯处理的卫星细胞培养物中几乎没有检测到蛋白降解活性。Northern印迹显示，卫星细胞培养上清液中可检测到编码基质胶原酶1的mRNA转录本的表达，佛波酯处理卫星细胞后，可增加基质胶原酶1转录产物的积累。相反，在卫星细胞中没有检测到编码基质金属蛋白酶-3的组成性或诱导性转录产物的表达。这些发现表明，卫星细胞可以合成和分泌特定的基质金属蛋白酶家族成员，提示骨骼肌细胞可能直接参与了肌肉发生和再生过程中细胞外基质的重塑。(C)1995年Wiley-Liss公司
The expression of matrix-degrading metalloproteases (MMPs) by human skeletal muscle satellite cells was investigated by zymography of cell culture media and by Northern blot analysis of mRNA prepared from satellite cells, Zymography in gelatin substrate gels revealed that satellite cells constitutively synthesize and secrete 72 kDa gelatinase (MMP-2), In addition, treatment of satellite cell cultures with phorbol ester resulted in an induction of 92 kDa gelatinase (MMP-9) activity, On casein substrate gels, little or no proteolytic activity was detectable in control or phorbol ester treated satellite cell cultures, suggesting that compared to fibroblasts, satellite cells secrete little or no interstitial collagenase (MMP-1) or stromelysin (MMP-3) activity, Northern blotting, however, revealed that there is detectable expression of mRNA transcripts encoding MMP-1 in satellite cell cultures, and that increased accumulation of MMP-1 mRNA transcripts occurs upon treatment of these cells with phorbol ester. In contrast, no constitutive, or induced expression of transcripts encoding MMP-3 was detectable in satellite cells. These findings show that satellite cells can synthesize and secrete selected members of the MMP family and suggest that skeletal muscle cells may participate directly in remodelling of the extracellular matrix during myogenesis and the regeneration of skeletal muscle. (C) 1995 Wiley-Liss, Inc.