Sensitive and rapid detection of freshwater crustacean Spiroplasmas by ISRs-sequence-targeted species-specific primers
Sensitive and rapid detection of freshwater crustacean Spiroplasmas by ISRs-sequence-targeted species-specific primers
复制标题
利用 ISR 序列靶向物种特异性引物灵敏快速检测淡水甲壳动物螺原体
DOI：
10.1007/s00217-008-0903-1
复制
发表时间：
2008-10-01
影响因子：
3.3
通讯作者：
Wang, Wen
中科院分区：
文献类型：
--
作者：
Bi, Keran;Gu, Wei;Wang, Wen
For the rapid and sensitive identification of the Spiroplasmas in the Chinese mitten crab Eriocheir sinensis, a species-specific PCR primer-pair was designed according to Spiroplasma sp. strain CRAB-specific segments of the previously sequenced 16S-23S rRNA intergenic spacer region (ISR). The PCR-mediated assay allowed identification of Spiroplasmas from different aquatic crustacean hosts, E. sinensis and red swamp crayfish Procambarus clarkia, in different geographical areas in China. No cross-reaction could be observed with other three available terrestrial plants/insects Spiroplasma strains (Spiroplasma sp. CH-1, Spiroplasma sp. CR-1 and Spiroplasma sp. M10). The sensitivity of detection of the Spiroplasma sp. strain CRAB could get as little as 100 fg of DNA template. The presented PCR method is useful for sensitive and specific detection of Spiroplasma from freshwater cultured crustaceans in the trade of aquatic products.
