Interactomic landscape of PA-X-chicken protein complexes of H5N1 influenza A virus
Interactomic landscape of PA-X-chicken protein complexes of H5N1 influenza A virus
复制标题
H5N1 甲型流感病毒 PA-X-鸡蛋白复合物的相互作用
DOI：
10.1016/j.jprot.2016.07.009
复制
发表时间：
2016-10-04
影响因子：
3.3
通讯作者：
Zhao, Guiping
中科院分区：
文献类型：
--
作者：
Li, Qinghe;Yuan, Xiaoya;Zhao, Guiping
作为一种新发现的由PA片段产生的异构体，也是病毒毒力的重要因素，对PA-X-宿主的相互作用知之甚少。在这里，我们介绍了H5N1甲型流感病毒(IAV)PA-X蛋白的相互作用图景，使用亲和纯化和质谱分析(AP-MS)产生的数据进行了描述。在鸡成纤维细胞中外源表达PA-X，AP-MS鉴定了PA-X相关蛋白复合体。使用高置信度的相互作用阈值，发现56种独特的蛋白质与PA-X有物理相互作用。PA-X相关宿主因子对包括Annexin和WD40结构域在内的特定蛋白结构域有较强的富集性。许多被描述为亲病毒或抗病毒宿主蛋白的蛋白质与PA-X相互作用，表明这些蛋白可能通过与PA-X的相互作用而在甲型流感病毒感染中发挥作用。本研究揭示了PA-X广泛的相互作用格局，为从病毒-宿主蛋白相互作用的角度进一步了解PA-X的功能奠定了基础。生物学意义：鉴定病毒-宿主相互作用蛋白对于全面了解病毒如何招募宿主细胞器以及宿主如何拮抗病毒感染至关重要。我们的研究揭示了PA-X的病毒-宿主相互作用组，并揭示了宿主蛋白与PA-X之间的相互作用，这可能在病毒感染中发挥关键作用。(C)2016年，由爱思唯尔出版。
As a newly identified isoform generated from the PA segment and an essential factor for viral virulence, little is known about PA-X-host interactions. Here we present the interactomic landscape of PA-X protein of H5N1 influenza A virus (IAV), described using data generated from affinity purification and mass spectrometry (AP-MS). PA-X was exogenously expressed in chicken fibroblast cells and PA-X associated protein complexes were identified by AP-MS. Using a high confidence threshold for interaction 56 unique proteins were found to have physical interactions with PA-X. PA-X associated host factors showed strong enrichment for specific protein domains, including annexin and WD40 domains. Many proteins that have been described as pro or antiviral host proteins interact with PA-X, indicating the possible effect of these proteins on influenza A viral infections facilitated by interactions with PA-X. This study has uncovered the comprehensive interactomic landscape of PA-X and laid the foundation for further understanding of PA-X function in terms of viral-host protein interactions.Biological significance: Identification of viral-host interacting proteins is vital for the comprehensive understanding of how virus recruits the host cellular machinery and how host antagonizes virus infection. Our study reveals the viral-host interactome of PA-X and uncovers interactions between host proteins and PA-X which might have crucial roles in viral infection. (C) 2016 Published by Elsevier B.V.