Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction.
Receptor component protein (RCP): a member of a multi-protein complex required for G-protein-coupled signal transduction.
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DOI：
10.1042/bst0300460
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发表时间：
2002-08
影响因子：
3.9
通讯作者：
M. A. Prado;B. Evans-Bain;I. Dickerson
中科院分区：
文献类型：
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作者：
M. A. Prado;B. Evans-Bain;I. Dickerson
降钙素基因相关肽 (CGRP) 受体成分蛋白 (RCP) 是一种由 148 个氨基酸组成的细胞内蛋白，是神经肽 CGRP 受体上 G 蛋白偶联信号转导所必需的。 RCP 与其他两种蛋白质一起构成功能性 CGRP 受体：降钙素受体样受体 (CRLR) 和受体活性修饰蛋白 1 (RAMP1)。 CRLR 具有 G 蛋白偶联受体的典型七跨膜拓扑结构；它需要 RAMP1 才能运输到细胞表面并实现配体特异性，并且需要 RCP 才能与细胞信号转导途径偶联。我们制作了表达 RCP 反义构建体的细胞系，并确定 CGRP 介导的信号转导减少，而 CGRP 结合不受影响。此外，另外两个内源性 G 蛋白偶联受体的信号传导未受影响，表明 RCP 对有限的受体子集具有特异性。
The calcitonin-gene-related peptide (CGRP) receptor component protein (RCP) is a 148-amino-acid intracellular protein that is required for G-protein-coupled signal transduction at receptors for the neuropeptide CGRP. RCP works in conjunction with two other proteins to constitute a functional CGRP receptor: calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein 1 (RAMP1). CRLR has the stereotypical seven-transmembrane topology of a G-protein-coupled receptor; it requires RAMP1 for trafficking to the cell surface and for ligand specificity, and requires RCP for coupling to the cellular signal transduction pathway. We have made cell lines that expressed an antisense construct of RCP and determined that CGRP-mediated signal transduction was reduced, while CGRP binding was unaffected. Furthermore, signalling at two other endogenous G-protein-coupled receptors was unaffected, suggesting that RCP was specific for a limited subset of receptors.