Simulation of triacylglycerol ion profiles: bioinformatics for interpretation of triacylglycerol biosynthesis
Simulation of triacylglycerol ion profiles: bioinformatics for interpretation of triacylglycerol biosynthesis
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DOI：
10.1194/jlr.m033837
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发表时间：
2013-04-01
影响因子：
6.5
通讯作者：
Han, Xianlin
中科院分区：
文献类型：
--
作者：
Han, Rowland H.;Wang, Miao;Han, Xianlin
虽然细胞内三酰甘油（TAG）的合成途径已得到很好的阐明，评估的贡献，在不同的哺乳动物器官，特别是在病理生理条件下的单个途径的TAG池，是困难的，虽然不是不可能的。在此，我们开发并验证了一种新的生物信息学方法，通过模拟鸟枪脂质组学确定的TAG离子谱来评估已知途径对TAG池的差异贡献。应用这种强大的方法来确定小鼠心脏，肝脏和骨骼肌中的这种贡献，并检查高脂饮食治疗后诱导的小鼠肝脏中这些途径的变化。这清楚地表明，通过模拟脂质组学数据，可以很容易地实现在病理生理条件下改变的TAG生物合成途径的评估。总的来说，这一新的发展应该大大促进我们对肥胖和脂毒性状态下TAG积累的生化机制的理解。汉河，巴西-地H、M. Wang，X. Fang和X.韩三酰甘油离子分布的模拟：用于解释三酰甘油生物合成的生物信息学。J. Lipid Res. 2013. 54：1023-1032。
Although the synthesis pathways of intracellular triacylglycerol (TAG) species have been well elucidated, assessment of the contribution of an individual pathway to TAG pools in different mammalian organs, particularly under pathophysiological conditions, is difficult, although not impossible. Herein, we developed and validated a novel bioinformatic approach to assess the differential contributions of the known pathways to TAG pools through simulation of TAG ion profiles determined by shotgun lipidomics. This powerful approach was applied to determine such contributions in mouse heart, liver, and skeletal muscle and to examine the changes of these pathways in mouse liver induced after treatment with a high-fat diet. It was clearly demonstrated that assessment of the altered TAG biosynthesis pathways under pathophysiological conditions can be readily achieved through simulation of lipidomics data. Collectively, this new development should greatly facilitate our understanding of the biochemical mechanisms underpinning TAG accumulation at the states of obesity and lipotoxicity.-Han, R. H., M. Wang, X. Fang, and X. Han. Simulation of triacylglycerol ion profiles: bioinformatics for interpretation of triacylglycerol biosynthesis. J. Lipid Res. 2013. 54: 1023-1032.