Vasculogenic mimicry and pseudo-comedo formation in breast cancer
Vasculogenic mimicry and pseudo-comedo formation in breast cancer
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DOI：
10.1002/ijc.10423
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发表时间：
2002-06-20
影响因子：
6.4
通讯作者：
Konishi, F
中科院分区：
文献类型：
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作者：
Shirakawa, K;Wakasugi, H;Konishi, F
肿瘤的生长和血行转移需要血液供应。直到最近，该领域的大多数研究都集中在血管生成的作用上，即将皮尤血管从预先存在的血管募集到肿瘤中。此前，在一项乳腺癌 (IBC) 研究中，我们使用源自 IBC 患者的已确定的炎性乳腺癌 (IBC) 异种移植物 (WIBC-9)（Shirakawa 等人，Cancer Res 2001:61:445-451），我们报告观察到血管生成拟态 (VM)，这是一种癌症组织内的血流不伴有内膜的情况 内皮细胞（EC）（Shirakawa 等人，Cancer Res 2002：62：560-566）。在本研究中，我们使用免疫组织化学和激光捕获显微切割 (LCM)，然后进行巢式逆转录酶聚合酶链反应 (RT-PCR)，检查了 331 例手术切除的乳腺癌标本，以寻找 VM 的证据。令人惊讶的是，331 个样本中的 7.9%（26 个样本）表现出 VM 的证据。在这 26 个 VM 标本中，84.6%（22 个标本）表现出假性粉刺形成。对 8 个显微解剖的典型 VM 标本的 RT-PCR 分析显示，分别有 63%、50%、0% 和 0% 的标本表达 Tie-2、Flt-1、凝血酶受体和 CD31。相比之下，8 个非 VM 肿瘤样本的 RT-PCR 分析结果显示这些基因的表达呈阴性。与 305 例非 VM 病例相比，26 例 VM 病例的血行复发率较高（p = 0.059），而 5 年生存率较低（p = 0.071）。然而，两组之间的肿瘤大小、淋巴结转移、雌激素受体或孕激素受体没有显着差异（p > 0.1）。我们的结果表明，VM 的存在增加了血行转移的可能性，并且与预后成反比。 (C) 2002 Wiley-Liss, Inc.
Tumors require a blood supply for growth and hematogenous metastases. Until recently, most research in this area has focused on the role of angiogenesis, the recruitment of pew vessels into a tumor from preexisting vessels. Previously, in a study of breast cancer (IBC), in which we used established inflammatory breast cancer (IBC) xenografts (WIBC-9) originating from a patient with IBC (Shirakawa et at., Cancer Res 2001:61:445-451), we reported observing vasculogenic mimicry (VM), a condition in which bloodstreams within cancer tissue are not accompanied by a lining of endothelial cells (ECs) (Shirakawa et al., Cancer Res 2002:62: 560-566). In the present study, we examined 331 surgically resected breast cancer specimens for evidence of VM, using immunohistochemistry and laser-captured microdissection (LCM) followed by nested reverse transcriptase polymerase chain reaction (RT-PCR). Surprisingly, 7.9% (26 specimens) of the 331 specimens exhibited evidence of VM. Of these 26 VM specimens, 84.6% (22 specimens) exhibited pseudocomedo formation. RT-PCR analysis of 8 microdissected typical VM specimens revealed expression of Tie-2, Flt-1, thrombin receptor and CD31 in 63, 50, 0 and 0% of specimens, respectively. In contrast, results of RT-PCR analysis of 8 specimens from non-VM tumors were negative for expression of these genes. The 26 VM cases tended to have a higher percentage of hematogenous recurrence (p = 0.059) and a lower percentage of 5-year survival (p = 0.071) than the 305 non-VM cases. However, there were no significant differences in tumor size, lymph node metastasis, estrogen receptors or progesterone receptors between the 2 groups (p > 0.1). Our results suggest that the existence of VM increases the likelihood of hematogenous metastases and is in inverse proportion to prognosis. (C) 2002 Wiley-Liss, Inc.