Localization of a family of MRNAS in a single cell type and its precursors in sea urchin embryos.
Localization of a family of MRNAS in a single cell type and its precursors in sea urchin embryos.
复制标题
MRNAS 家族在单细胞类型中的定位及其在海胆胚胎中的前体。
DOI：
10.1073/pnas.80.9.2656
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Angerer,RC
中科院分区：
文献类型：
--
作者：
Lynn,DA;Angerer,LM;Bruskin,AM;Klein,WH;Angerer,RC
在早期海胆发育过程中，每个胚胎的Spec 1 mRNA丰度增加了100倍。以前的研究表明，这种mRNA的富集在原肠胚和plutei的外胚层组分。我们已经确定了这种mRNA的精确定位，通过原位杂交技术。在pluteus幼虫中，mRNA高度限制于胚胎背部的一组形态均匀的外胚层细胞。mRNA在外胚层的其他区域或内胚层和中胚层中检测不到。定位的模式已经建立在原肠胚阶段，在这些细胞是可区分的形态标准。Spec 1 mRNA的这种分布模式与大量poly（A）+ mRNA的分布模式不同。每个胚胎的Spec 1 mRNA的量和含有这种RNA的细胞的数量的测量表明，在pluteus阶段每个细胞大约有500个Spec 1 mRNA分子，在原肠胚阶段可能是其两倍。这些结果表明，原位杂交方法的灵敏度允许检测包含约等于0.05%的胚胎mRNA核苷酸的序列。
Spec 1 mRNAs increase 100-fold in abundance per embryo during early sea urchin development. Previous studies indicated an enrichment of this mRNA in ectoderm fractions of gastrulae and plutei. We have determined the precise localization of this mRNA by in situ hybridization techniques. In pluteus larvae, the mRNA is highly restricted to a set of morphologically uniform ectoderm cells in the dorsal part of the embryo. The mRNA is not detectable in other regions of ectoderm or in endoderm and mesoderm. The pattern of localization is already established at the gastrula stage, before these cells are distinguishable by morphological criteria. This pattern of distribution of Spec 1 mRNA is distinct from that of bulk poly(A)+ mRNA. Measurements of the amount of Spec 1 mRNA per embryo and the number of cells containing this RNA indicate that there are about 500 Spec 1 mRNA molecules per cell at the pluteus stage and probably twice as many at the gastrula stage. These results indicate that the sensitivity of the in situ hybridization method allows detection of sequences that comprise approximately equal to 0.05% of the embryo mRNA nucleotides.