Systematic microRNAome profiling reveals the roles of microRNAs in milk protein metabolism and quality: insights on low-quality forage utilization.
Systematic microRNAome profiling reveals the roles of microRNAs in milk protein metabolism and quality: insights on low-quality forage utilization.
复制标题
系统的MicroRNAOME分析揭示了microRNAS在牛奶蛋白代谢和质量中的作用：低质量饲料利用的见解。
DOI：
10.1038/srep21194
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发表时间：
2016-02-17
影响因子：
4.6
通讯作者：
Guan le L
中科院分区：
文献类型：
--
作者：
Wang D;Liang G;Wang B;Sun H;Liu J;Guan le L
在这项研究中，我们通过研究高品质和低品质日粮奶牛参与蛋白质合成和代谢的五个关键代谢组织的 miRNA 组，研究了奶牛乳蛋白生产的分子调控机制。总共有 340、338、337、330 和 328 个 miRNA 在瘤胃、十二指肠、空肠、肝脏和乳腺组织中表达。一些 miRNA 与饲料和氮效率高度相关，靶基因参与氨基酸 (AA) 的运输和磷酸化。此外，低质量饲料日粮（玉米秸秆和稻草）影响饲料和氮效率相关 miRNA 的表达，例如瘤胃中的 miR-99b、十二指肠中的 miR-2336、空肠中的 miR-652、肝脏中的 miR-1 和乳腺中的 miR-181a。预计瘤胃 miR-21-3p 和肝脏 miR-2285f 分别通过靶向 ATP1A2 和 SLC7A8 来调节 AA 运输。此外，牛特异性 miRNA 调节瘤胃上皮的增殖和形态，以及肝脏脂质和支链 AA 的代谢，揭示了牛特异性机制。我们的结果表明，在这五个组织中表达的 miRNA 在调节下游产奶中 AA 的运输中发挥着作用，这可能是与低质量饲料下的低乳蛋白有关的重要机制。
In this study, we investigated the molecular regulatory mechanisms of milk protein production in dairy cows by studying the miRNAomes of five key metabolic tissues involved in protein synthesis and metabolism from dairy cows fed high- and low-quality diets. In total, 340, 338, 337, 330, and 328 miRNAs were expressed in the rumen, duodenum, jejunum, liver, and mammary gland tissues, respectively. Some miRNAs were highly correlated with feed and nitrogen efficiency, with target genes involved in transportation and phosphorylation of amino acid (AA). Additionally, low-quality forage diets (corn stover and rice straw) influenced the expression of feed and nitrogen efficiency-associated miRNAs such as miR-99b in rumen, miR-2336 in duodenum, miR-652 in jejunum, miR-1 in liver, and miR-181a in mammary gland. Ruminal miR-21-3p and liver miR-2285f were predicted to regulate AA transportation by targeting ATP1A2 and SLC7A8, respectively. Furthermore, bovine-specific miRNAs regulated the proliferation and morphology of rumen epithelium, as well as the metabolism of liver lipids and branched-chain AAs, revealing bovine-specific mechanisms. Our results suggest that miRNAs expressed in these five tissues play roles in regulating transportation of AA for downstream milk production, which is an important mechanism that may be associated with low milk protein under low-quality forage feed.