ISOLATION OF VIRUSES FROM FEMALE CULEX-TRITAENIORHYNCHUS IN AEDES-ALBOPICTUS CELL-CULTURES
ISOLATION OF VIRUSES FROM FEMALE CULEX-TRITAENIORHYNCHUS IN AEDES-ALBOPICTUS CELL-CULTURES
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DOI：
10.4269/ajtmh.1981.30.449
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发表时间：
1981-01-01
影响因子：
3.3
通讯作者：
FUKAI, K
中科院分区：
文献类型：
--
作者：
IGARASHI, A;BUEI, K;FUKAI, K
用白纹伊蚊克隆C6/36细胞和乳鼠脑（SMB）接种的方法分离了雌性三带喙库蚊的病毒。从1978年夏季在日本大阪府采集的349个池中共处理了32,812只蚊子。在C6/36细胞中分离到39株乙型脑炎病毒（JEV），而在SMB细胞中分离到27株。24个池在细胞培养和SMB中均产生JEV， 10个池仅在细胞培养中产生JEV， 3个池仅在SMB中产生JEV。在细胞培养中分离到2株Getah （GET）病毒，但在SMB中未分离到。与28度时C6/36细胞上的斑块形成滴度相比，GET菌株23个斑块分离株中的2个在BHK21（鼠肾）细胞或SMB-LD50上的斑块形成滴度显著降低。C.在C6/36细胞上通过斑块形成检测到许多未知的滤过剂。
Isolation of viruses from female C. tritaeniorhynchus was performed by inoculation of specimens into A. albopictus clone C6/36 cells and into suckling mouse brains (SMB). Altogether, 32,812 mosquitoes in 349 pools were processed from the specimens collected in Osaka Prefecture, Japan, during the summer of 1978. Thirty-nine strains of Japanese encephalitis virus (JEV) were isolated in C6/36 cells in contrast to 27 strains in SMB. Twenty-four pools yielded JEV both in cell cultures and in SMB, 10 yield JEV only in cell cultures and 3 only in SMB. Two strains of Getah (GET) virus were isolated in cell cultures but not in SMB. Two of the 23 plaque isolates of a GET strain showed significantly lower titers of plaque formation on BHK21 (hamster kidney) cells or SMB-LD50, compared with the titer of plaque formation on C6/36 cells at 28.degree. C. Many unidentified filterable agents were detected by plaque formation on C6/36 cells.