RNA editing and alternative splicing:: the importance of co-transcriptional coordination
RNA editing and alternative splicing:: the importance of co-transcriptional coordination
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DOI：
10.1038/sj.embor.7400621
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发表时间：
2006-03-01
期刊：
影响因子：
7.7
通讯作者：
Öhman, M
中科院分区：
文献类型：
--
作者：
Laurencikiene, J;Källman, AM;Öhman, M
RNA聚合酶II大亚基（pol II）的羧基末端结构域（CTD）对于几种共转录前信使RNA加工事件（包括加帽、3 '端加工和剪接）是必需的。我们研究了RNA pol II的CTD在ADAR 2（阿达尔：作用于RNA的腺苷脱氨酶）前体mRNA的A至I编辑和剪接协调中的作用。通过ADAR 2腺苷脱氨酶对Adar 2内含子4的自动编辑与剪接紧密耦合，因为二核苷酸AA至AI的修饰产生了新的3'剪接位点。与其他内含子不同，CTD对于内含子4在正常3'剪接位点或通过编辑产生的替代位点处的有效剪接是不需要的。然而，CTD是ADAR 2内含子4的有效共转录自动编辑所必需的。我们的研究结果暗示CTD在ADAR 2的位点选择性RNA编辑和编辑与选择性剪接的协调。
The carboxy-terminal domain (CTD) of the large subunit of RNA polymerase II (pol II) is essential for several co-transcriptional pre-messenger RNA processing events, including capping, 3'-end processing and splicing. We investigated the role of the CTD of RNA pol II in the coordination of A to I editing and splicing of the ADAR2 (ADAR: adenosine deaminases that act on RNA) pre-mRNA. The auto-editing of Adar2 intron 4 by the ADAR2 adenosine deaminase is tightly coupled to splicing, as the modification of the dinucleotide AA to AI creates a new 3' splice site. Unlike other introns, the CTD is not required for efficient splicing of intron 4 at either the normal 3' splice site or the alternative site created by editing. However, the CTD is required for efficient co-transcriptional auto-editing of ADAR2 intron 4. Our results implicate the CTD in site-selective RNA editing by ADAR2 and in coordination of editing with alternative splicing.