On the relationship of matrix association and DNA replication.
On the relationship of matrix association and DNA replication.
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DOI：
10.1615/critreveukargeneexpr.v10.i1.100
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发表时间：
2000
影响因子：
1.6
通讯作者：
B. P. Brylawski;S. Cohen;M. Cordeiro‐Stone;M. Schell;D. Kaufman
中科院分区：
文献类型：
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作者：
B. P. Brylawski;S. Cohen;M. Cordeiro‐Stone;M. Schell;D. Kaufman
核基质被认为含有蛋白质复合体的组装部位，这些蛋白质复合体催化DNA复制的启动和DNA的延长。为了探讨这种关系，我们提纯了人成纤维细胞在S期初复制的DNA，并将其用于构建粘粒文库。与一组克隆(约占该文库中总克隆的六分之一)的杂交研究表明，其中许多克隆在从早期复制的DNA以及核基质相关DNA制备的探针中突出显示。统计分析表明，这些杂交结果之间呈正相关。我们试图确定在人类细胞周期的S阶段早期激活的复制起源。因此，从这个文库中分离出来的克隆正在被分析，以寻找在其他复制起点中发现的结构基序的存在，以及潜在的连接到核基质的位置。这种分析方法在这里用已发表的人类层蛋白B2和HPRT基因复制起点的序列来说明。
The nuclear matrix is believed to contain sites of assembly of protein complexes that catalyze the initiation of DNA replication as well as DNA elongation. To explore this relationship, DNA replicated by human fibroblasts at the beginning of the S phase was purified and used to construct a cosmid library. Hybridization studies with a subgroup of clones (about one-sixth of the total clones in this library) showed that many of them were highlighted by probes prepared from early replicating DNA, as well as from nuclear matrix-associated DNA. Statistical analysis showed a positive correlation between these hybridization results. We seek to identify origins of replication that are activated early in the S phase of the cell cycle in human cells. Therefore, clones isolated from this library are being analyzed for the presence of structural motifs that have been found in other origins of replication and for potential sites of attachment to the nuclear matrix. This method of analysis is illustrated here using the published sequences for the origins of replication reported for the human lamin B2 and HPRT genes.