Thiol/Acrylate-Modified PEO-PPO-PEO Triblocks Used as Reactive and Thermosensitive Copolymers
Thiol/Acrylate-Modified PEO-PPO-PEO Triblocks Used as Reactive and Thermosensitive Copolymers
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DOI：
10.1021/bm800573e
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发表时间：
2008-10-01
影响因子：
6.2
通讯作者：
Yang, Huai
中科院分区：
文献类型：
--
作者：
Niu, Guoguang;Zhang, Hongbin;Yang, Huai
基于硫醇与丙烯酸酯封端的热敏泊洛沙姆188在生理条件下发生硫醇-烯反应，设计了结合了可逆热敏性和温和反应性的反应性热敏水凝胶。通过H-1 NMR、FT-IR、GPC、DSC、Ellman法和流变仪对修饰后的P188A、P188SH及其反应性进行了表征。研究发现硫醇-烯反应对pH和热敏感。在 37.0 摄氏度和 pH 7.4 的前 30 分钟内，有 77.7% 的 SH 参与了反应。大多数分子以C=C/SH摩尔比为1.5进行反应。硫醇-烯放热反应温和，变化约为 Delta H = -91.18 J/g。多嵌段或网络结构限制了水凝胶的溶解，相应地凝胶的持续时间和亚甲基蓝的释放时间延长至124小时。实验结果表明反应性热敏水凝胶在药物输送、组织工程和细胞封装方面的潜在应用。
The reactive thermal-sensitive hydrogels, which combined the reversible thermosensitive and mild reactive property, were designed based on thiol-ene reaction in physiological conditions between thiol and acrylate capped thermosensitive Poloxamer 188. The modified P188A, P188SH, and their reactivity were characterized by H-1 NMR, FT-IR, GPC, DSC, Ellman method, and Rheometer. It was found that the thiol-ene reaction was pH and thermal-sensitive. There was 77.7% SH involved into the reaction at 37.0 degrees C and pH 7.4 within the first 30 min. The most of molecules reacted as C=C/SH mol ratio was 1.5. The exothermic thiol-ene reaction was mild, with about Delta H = -91.18 J/g changes. The multiblocks or network structure limited the dissolution of hydrogel, correspondingly the gel's duration and the release time of methylene blue were prolonged to 124 h. The experimental results indicated the reactive thermal-sensitive hydrogel's potential applications in drug delivery, tissue engineering, and cell encapsulation.