Use of chitosan-clay composite as immobilization support for improved activity and stability of β-glucosidase
Use of chitosan-clay composite as immobilization support for improved activity and stability of β-glucosidase
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DOI：
10.1016/j.bej.2007.01.003
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发表时间：
2007-07-01
影响因子：
3.9
通讯作者：
Juang, Ruey-Shin
中科院分区：
文献类型：
--
作者：
Chang, Min-Yun;Juang, Ruey-Shin
将等重量的海螵蛸壳聚糖和活性粘土混合并制备成湿（未冷冻干燥）和干（冷冻干燥）珠粒。然后将所得复合珠与戊二醛交联作为固定化β-葡萄糖苷酶的载体。对固定化条件进行了优化，包括酶的负载量、固定化时间、交联时间、戊二醛用量等。比较了干、湿复合材料固定化酶的性能。结果表明，固定化酶的贮存稳定性高于游离酶。重复使用50次后，干燥复合物固定化酶的活性高于湿壳聚糖珠和湿复合物。研究了游离酶和固定化酶的pH稳定性和热稳定性。(c)2007 Elsevier B. V.保留所有权利。
Equal weights of cuttlebone chitosan and activated clay were mixed and prepared as the wet (without freeze-drying) and dried (with freeze-drying) beads. The resulting composite beads were then cross-linked with glutaraldehyde as the supports for P-glucosidase immobilization. The immobilization conditions including enzyme loading, immobilization time, cross-linking time, and the amount of glutaraldehye were optimized. The properties of the enzyme immobilized on wet and dried composites were compared. It was shown that the storage stability of immobilized enzyme was higher than that of free enzyme. The activity of immobilized enzyme on dried composite was higher those on wet chitosan beads and wet composite after being repeatedly used for 50 times. The pH and thermal stabilities of free and immobilized enzymes were also investigated. (c) 2007 Elsevier B.V. All rights reserved.