Protofibrils within fibrin fibres are packed together in a regular array
Protofibrils within fibrin fibres are packed together in a regular array
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DOI：
10.1055/s-0037-1613569
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发表时间：
2003-04-01
影响因子：
6.7
通讯作者：
Arcovito, G
中科院分区：
文献类型：
--
作者：
Caracciolo, G;De Spirito, M;Arcovito, G
本文用X射线能谱衍射技术研究了由人α-凝血酶活化的纤维蛋白原溶液中生长的纤维蛋白纤维的内部结构。实验表明，充分表征的22.5 nm的重复距离，这表明在纤维的纵向方向上的高秩序的原纤维排列的证据。衍射图还显示出在18.1 nm处的另一显著峰（以及在18.1/root 2处的其二级反射），表明在纤维蛋白中存在高度的横向级。报道的结果直接证实，在未受干扰的湿样品，原纤维密切相关，产生结晶轴向和赤道包装根据多束模型的结论。
The inner structure of fibrin fibres grown from fibrinogen solution activated by human alpha-thrombin was investigated by means of an Energy Dispersive X-ray Diffraction technique. The experiments show evidence for the well-characterized 22.5 nm repeat distance, which indicates the high order of protofibril arrangement in the longitudinal direction of fibres. The diffraction pattern also manifested a further pronounced peak at 18.1 nm (and its second order reflection at 18.1/root2) demonstrating the existence in fibrin of a high degree of lateral order. The reported results directly confirm, on unperturbed wet samples, that protofibrils closely associate giving rise to a crystalline axial and equatorial packing according to the conclusions of the multibundle model.