Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library
Novel in vitro screening system for monoclonal antibodies using hypermutating chicken B cell library
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DOI：
10.1263/jbb.102.478
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发表时间：
2006-11-01
影响因子：
2.8
通讯作者：
Ohmori, Hitoshi
中科院分区：
文献类型：
--
作者：
Todo, Kagefumi;Miyake, Kenji;Ohmori, Hitoshi
在这里，我们报告了一种使用超突变鸡B细胞系DT40-SW的单克隆抗体的体外筛选系统。当开启高突变时，培养的DT40-SW细胞构成一个抗体文库，从中成功分离出分泌针对测试抗原的抗体的克隆，并通过关闭突变机制来实现遗传稳定。
Here, we report an in vitro screening system for monoclonal antibodies using a hypermutating chicken B cell line, DT40-SW. When switching on hypermutation, cultured DT40-SW cells constituted an antibody library, from which clones secreting antibodies to a test antigen were successfully isolated, and genetically stabilized by switching off the mutation machinery.