Sealing Effect of Cross-Linked Gelatin Glue in the Rat Lung Air Leak Model
Sealing Effect of Cross-Linked Gelatin Glue in the Rat Lung Air Leak Model
复制标题
交联明胶对大鼠肺漏气模型的密封作用
DOI：
10.1016/j.athoracsur.2016.02.038
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Shigeki Taniguchi
中科院分区：
文献类型：
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作者：
Norikazu Kawai;Shuko Suzuki;Hiroshi Naito;Keiji Kushibe;Takashi Tojo;Yoshito Ikada;Shigeki Taniguchi
背景漏气是肺外科手术中常见的问题。在这项研究中，我们评估的有效性和安全性的明胶胶（交联与戊二醛）在大鼠模型的肺airleak.MethodsA模型的肺瘘，在大鼠肺与使用针。然后用明胶胶（A组）、纤维蛋白胶（B组）或纤维蛋白胶与聚乙醇酸片（C组）密封瘘管。测量各组的密封破坏压力，并对结果进行比较。为了评估明胶胶的生物相容性，用切口创建肺损伤模型，并应用明胶胶密封伤口。结果A组的密封破坏压力（47.88 ± 6.69 mm Hg）明显高于B组（24.67 ± 3.24 mm Hg，p= 0.0302）或C组（28.67 ± 3.55 mm Hg，p= 0.0406）。组织学上，明胶胶牢固地粘附在肺表面，只有局部组织炎症observed.ConclusionsThe密封效果的明胶胶是上级的纤维蛋白胶，有或没有聚乙醇酸片。此外，明胶胶仅引起肺部轻度炎症，并被吸收，无任何不良异物反应。这些发现表明，明胶胶可能是一种治疗有效的生物材料，用于密封肺伤口和恢复呼吸功能。
BackgroundAir leak is a common problem in pulmonary surgical procedures. In this study, we evaluated the efficacy and safety of gelatin glue (cross-linked with glutaraldehyde) in a rat model of lung air leak.MethodsA model of pulmonary fistula was created in the rat lung with the use of a needle. The fistula was then sealed with either gelatin glue (group A), fibrin glue (group B), or fibrin glue with a polyglycolic acid sheet (group C). The seal breaking pressures were measured for each group, and the results were compared. To assess the biocompatibility of the gelatin glue, a model of lung damage was created with incision, and the gelatin glue was applied to seal the wound. Histologic analysis was then performed on the lung tissue.ResultsThe seal breaking pressure in group A (47.88 ± 6.69 mm Hg) was significantly higher than that in group B (24.67 ± 3.24 mm Hg,p= 0.0302) or group C (28.67 ± 3.55 mm Hg,p= 0.0406). Histologically, the gelatin glue adhered firmly to the lung surface, and only localized tissue inflammation was observed.ConclusionsThe sealing effect of gelatin glue was superior to that of fibrin glue, with or without a polyglycolic acid sheet. In addition, the gelatin glue only caused mild inflammation of the lung and was absorbed without any adverse foreign body response. These findings suggest that gelatin glue may be a therapeutically effective biomaterial for sealing lung wounds and restoring respiratory function.