MURINE TROPHOBLAST FAILURE AND SPONTANEOUS-ABORTION
MURINE TROPHOBLAST FAILURE AND SPONTANEOUS-ABORTION
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DOI：
10.1111/j.1600-0897.1993.tb00587.x
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发表时间：
1993-05-01
影响因子：
3.6
通讯作者：
CROY, BA
中科院分区：
文献类型：
--
作者：
CLARK, DA;BANWATT, D;CROY, BA
问题：有人提出感染会引发流产，而病毒在小鼠自发吸收中的作用尚未得到测试。方法：从与 DBA/2J 交配后的早晨开始，给 CBA/J 和 C3H/HeJ 雌性小鼠喂食抗病毒药物利巴韦林（1-β-D-呋喃核糖基-1,2,4-三唑-3-3-甲酰胺） 结果：利巴韦林治疗增加了第 13.5 天的流产（吸收）率，这与胚胎发育速度迟缓和滋养层发育不全有关。滋养层依赖的蜕膜相关的可溶性抑制活性降低，但没有报告的与半同种异体和异种小鼠胚胎吸收相关的母体单核细胞浸润类型。这可能是由于利巴韦林的免疫抑制作用。利巴韦林能够在体外有效抑制小鼠滋养层细胞和肥大细胞瘤细胞系的增殖。结论：有几种药物诱导的小鼠流产模型为了解自发性妊娠失败的潜在机制提供了有用的见解，但在利巴韦林模型中，滋养层发育的直接损害似乎是造成这种情况的原因。
PROBLEM: Infection has been proposed to initiate abortion, and the role of viruses in spontaneous resorption in mice has not been tested.METHOD: The anti-viral drug ribavirin (1-beta-D-ribofuranosyl-1,2,4-triazole-3-3-carboxamide) was fed to CBA/J and C3H/HeJ female mice beginning on the morning after mating with DBA/2J males.RESULTS: Ribavirin treatment increased the rate of abortion (resorption) on day 13.5, and this was associated with retardation of the rate of embryo development and hypoplasia of the trophoblast. There was a reduction in trophoblast-dependent decidua-associated soluble suppressor activity, but there was no maternal mononuclear cell infiltrate of the type reported in association with resorption of semiallogeneic and xenogeneic mouse embryos. This may be due to an immunosuppressive effect of ribavirin. Ribavirin was able to potently suppress proliferation of mouse trophoblast and mastocytoma cell lines in vitro.CONCLUSIONS: There are several drug-induced murine abortion models that provide useful insights into potential mechanisms underlying spontaneous pregnancy failure, but in the ribavirin model, a direct impairment of trophoblast development appears to be responsible.