Ascorbic acid is decreased in induced sputum of mild asthmatics
Ascorbic acid is decreased in induced sputum of mild asthmatics
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DOI：
10.1080/08958370390168184
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发表时间：
2003-02-01
影响因子：
2.1
通讯作者：
Alexis, N
中科院分区：
文献类型：
--
作者：
Kongerud, J;Crissman, K;Alexis, N
哮喘主要是一种气道炎症性疾病，支气管气道已被证明特别容易受到氧化剂诱导的组织损伤。抗氧化剂抗坏血酸（AA）在防御气道中的氧化剂攻击中起着至关重要的作用。据报道，哮喘患者血浆和BAL液中AA水平降低，但在直接接近哮喘病理的部位（支气管气道）中没有。我们调查了哮喘患者与健康受试者相比，气道中AA水平是否不足。我们在一组轻度哮喘患者（n = 16）和健康对照组（n = 18）中进行诱导痰（IS），以比较这两组气道中抗氧化剂的组成水平。我们报告说，与正常人相比，哮喘患者IS样本的细胞（17 +/- 3 ng/10（6）cells vs. 40 +/- 4 ng/10（6）cells）和液相部分（616 +/- 152 ng/ml vs. 937 +/- 161 ng/ml）中AA显著降低。谷胱甘肽（GSH）和α-生育酚无差异。这些结果表明AA缺乏可能是哮喘病理生理学的潜在因素或对哮喘气道炎症的反应。
Asthma is primarily an airways inflammatory disease, and the bronchial airways have been shown to be particularly susceptible to oxidant-induced tissue damage. The antioxidant ascorbic acid (AA) plays an essential role in defending against oxidant attack in the airways. Decreased levels of AA have been reported in the plasma and BAL fluid of asthmatics, but not at the site directly proximal to asthma pathology, the bronchial airways. We investigated whether asthmatics have deficient levels of AA in the airways compared to healthy subjects. We performed induced sputum (IS) in a group of mild asthmatics (n = 16) and healthy controls (n = 18) in order to compare constitutive levels of antioxidants in the airways of these two groups. We report that asthmatics had significantly decreased AA in both the cellular (17 +/- 3 ng/10(6) cells vs. 40 +/- 4 ng/10(6) cells) and fluid-phase fraction (616 +/- 152 ng/ml vs. 937 +/- 161 ng/ml) of the IS sample compared to normals. No differences were found with glutathione (GSH) and alpha-tocopherol. These results suggest that AA deficiency may be either an underlying factor in the pathophysiology of asthma or a response to asthmatic airways inflammation.