Protein engineering for metabolic engineering: current and next-generation tools.
Protein engineering for metabolic engineering: current and next-generation tools.
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DOI：
10.1002/biot.201200371
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发表时间：
2013-05
影响因子：
4.7
通讯作者：
Liao, James C.
中科院分区：
文献类型：
--
作者：
Marcheschi, Ryan J.;Gronenberg, Luisa S.;Liao, James C.
代谢工程背景下的蛋白质工程对工业生物技术领域越来越重要。随着对生物生产的食品、燃料、化学品、食品添加剂和药物的需求持续增长，将需要设计和修饰蛋白质以实现新功能的能力，以满足工程生物体代谢的高生产率需求。本文综述了蛋白质的选择、建模和工程改造以提高或改变其活性的研究进展。一些方法只是最近才被开发用于一般用途，并刚刚开始在代谢工程界找到更大的应用。我们还讨论了在蛋白质中产生随机和有针对性的多样性以产生用于分析的突变体库的方法。最近使用这些技术来改变辅因子的使用，产生非天然氨基酸，醇，羧酸，并改变生物体表型的成功工程蛋白质的代谢工程目的的例子，并进行了讨论。
Protein engineering in the context of metabolic engineering is increasingly important to the field of industrial biotechnology. As the demand for biologically-produced food, fuels, chemicals, food additives, and pharmaceuticals continues to grow, the ability to design and modify proteins to accomplish new functions will be required to meet the high productivity demands for the metabolism of engineered organisms. This article reviews advances of selecting, modeling, and engineering proteins to improve or alter their activity. Some of the methods have only recently been developed for general use and are just beginning to find greater application in the metabolic engineering community. We also discuss methods of generating random and targeted diversity in proteins to generate mutant libraries for analysis. Recent uses of these techniques to alter cofactor use, produce non-natural amino acids, alcohols, and carboxylic acids, and alter organism phenotypes are presented and discussed as examples of the successful engineering of proteins for metabolic engineering purposes.
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