MECHANISM OF ACTIVATION OF AN N-RAS GENE IN THE HUMAN FIBRO-SARCOMA CELL-LINE HT1080
MECHANISM OF ACTIVATION OF AN N-RAS GENE IN THE HUMAN FIBRO-SARCOMA CELL-LINE HT1080
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DOI：
10.1002/j.1460-2075.1984.tb01970.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
HALL, A
中科院分区：
文献类型：
--
作者：
BROWN, R;MARSHALL, CJ;HALL, A
A full length [oncogene] N-ras gene was cloned from both the human fibrosarcoma cell line HT1080 and from normal human DNA. N-ras isolated from HT1080 will efficiently induce morphological transformation of NIH/3T3 cells in a transfection assay, whereas N-ras isolated from normal human DNA has no effect on NIH/3T3 cells. The coding regions of the normal N-ras gene were sequenced and the predicted amino acid sequence of the N-ras product is very similar to that of the c-Ha-ras1 and c-Ki-ras2 products. By making chimeric molecules between the 2 cloned genes the activating alteration in the HT1080 N-ras gene was localized to a single base change that results in an amino acid alteration at position 61 of the p21 N-ras product.
