Comparison of the positive and negative ion electrospray mass spectra of some small peptides containing pyroglutamate
Comparison of the positive and negative ion electrospray mass spectra of some small peptides containing pyroglutamate
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DOI：
10.1002/rcm.576
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发表时间：
2002-01-01
影响因子：
2
通讯作者：
Baudinette, RV
中科院分区：
文献类型：
--
作者：
Boontheung, P;Brinkworth, CS;Baudinette, RV
所选含焦谷氨酸的肽的[M+H]（+）的正离子电喷雾质谱和[M-H]（-）离子的负离子电喷雾质谱均提供测序数据。负离子光谱显示除了由Glu和Phe残基的侧链引发的δ和γ主链裂解之外，还存在正常的α和β主链裂解。例如，pGlu Pro Gln瓦尔Phe Val-NH 2的[M-H]（-）离子在m/z 224（delta，Gln 3）、244（gamma，Phe 4）、451（delta，Phe 4）、471（gamma，Gln 3）处显示δ和γ峰。一些负离子光谱显示出不寻常的孙女峰，其起源于β（1）裂解离子的α和β或δ和γ主链裂解。版权所有（C）2002约翰威利父子有限公司
The positive ion electrospray mass spectra of [M+H](+) and the negative ion electrospray mass spectra of [M-H](-) ions of selected pyroglutamate containing peptides both provide sequencing data. The negative ion spectra show the normal alpha and beta backbone cleavages in addition to delta and gamma backbone cleavages initiated by the side chains of Glu and Phe residues. For example, the [M-H](-) ion of pGlu Pro Gln Val Phe Val-NH2 shows delta and gamma peaks at m/z 224 (delta, Gln3),244 (gamma, Phe4),451 (delta, Phe4), 471 (gamma, Gln3). Some of the negative ion spectra show unusual grandaughter peaks that originate by alpha and beta, or delta and gamma backbone cleavages of a beta(1) cleavage ion. Copyright (C) 2002 John Wiley Sons, Ltd.