Characterization of long-term functional outcome in a murine model of mild brain ischemia
Characterization of long-term functional outcome in a murine model of mild brain ischemia
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DOI：
10.1016/j.jneumeth.2012.12.021
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发表时间：
2013-03-15
影响因子：
3
通讯作者：
Endres, Matthias
中科院分区：
文献类型：
--
作者：
Balkaya, Mustafa;Kroeber, Jan;Endres, Matthias
在缺血后几周内评估功能结果是提高实验性脑卒中研究临床相关性的关键组成部分。通过一系列行为测试，我们描述了大脑中近端动脉闭塞（MCAo） 30分钟后4周内小鼠的功能结果。我们评估了旋转杆、烟囱、杆和圆柱试验，以评估主要在纹状体诱发梗死的短暂性卒中模型的短期功能缺陷。对角试验、去胶试验、圆柱体试验、走步试验、爪偏向试验和新颖旋转试验进行长期功能预后评价。Rotarod在第一周内检测到缺陷，杆子测试在MCAo后的中间时间点是可靠的。角测试、去除粘合剂测试、走步和爪子偏好测试在长达4周的时间里检测到缺陷，新颖的角旋转和碗测试也是如此。烟囱和圆筒试验在我们的轻度中风模型中没有被证明是有用的。总之，我们建立了极测试和旋转棒作为评估轻度中风后早期感觉运动缺陷的有用工具，以及角测试和黏合剂去除测试在后期的时间点。另外，转角旋转可能是长期功能的合适测试。对于临床相关的缺陷，测试电池可以进一步辅以t台和爪子偏好测试。行为结果与28天的病变大小没有相关性，并且确定这些测试是否有助于检测神经保护或再生治疗的潜在益处需要进一步的测试。(c) 2012 Elsevier B.V.版权所有
Evaluation of functional outcome over the course of several weeks after ischemia is a key component in improving the clinical relevance of experimental stroke studies. Using a battery of behavioral tests, we characterized functional outcome in mice over 4 weeks following 30 min of proximal middle cerebral artery occlusion (MCAo). We evaluated rotarod, chimney, pole and cylinder tests to assess short term functional deficits in a transient stroke model which induces infarcts mainly in the striatum. The corner test, adhesive removal test, cylinder test, catwalk, paw preference test and novel tests of rotation were evaluated for long-term functional outcome. Rotarod detected deficits within the first week and pole test was reliable up to intermediate time points after MCAo. Corner test, adhesive removal test, catwalk and paw preference test detected deficits for up to 4 weeks, as did the novel corner rotation and bowl tests. Chimney and cylinder test did not prove useful in our model of mild stroke. In summary, we established the pole test and rotarod as useful tools to evaluate sensory motor deficits early after mild stroke, and corner test and adhesive removal test at later time-points. Alternatively, corner rotation may be a suitable test of long-term function. Test batteries may be further complemented by catwalk and paw preference test for clinically relevant deficits. There was no correlation of behavioral outcome with lesion size at 28 days, and determining whether these tests are useful for detecting a potential benefit of neuroprotective or regenerative therapies requires further testing. (c) 2012 Elsevier B.V. All rights reserved.