Gene expression markers for Caenorhabditis elegans vulval cells
Gene expression markers for Caenorhabditis elegans vulval cells
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DOI：
10.1016/s0925-4773(03)00117-5
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发表时间：
2002-12-01
影响因子：
2.6
通讯作者：
Sternberg, Paul W.
中科院分区：
文献类型：
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作者：
Inoue, Takao;Sherwood, David R.;Sternberg, Paul W.
秀丽隐杆线虫外阴发育过程中细胞命运模式的分析主要依赖于细胞分裂和细胞运动的直接观察（细胞谱系分析）。然而，从EM连续切片重建发育中的外阴提出了七种不同的细胞类型（vulA，vulB 1，vulB 2，vulC，vulD，vulE和vulF），其中许多不能根据这些观察结果进行区分。在此，我们报告了7个基因，egl-17，cdh-3，ceh-2，zmp-1，B0034.1，T04 B2.6和F47 B8.6的外阴表达的gfp，cfp和yfp（绿色荧光蛋白和颜色变体）报告融合。每个基因在外阴细胞的特定子集中表达，因此可用作外阴细胞命运的标记。总之，标志物的表达区分六种细胞类型，并揭示了发育中外阴基因表达的严格时间控制。(c)2003爱思唯尔科学爱尔兰有限公司保留所有权利。
The analysis of cell fate patterning during the vulval development of Caenorhabditis elegans has relied mostly on the direct observation of cell divisions and cell movements (cell lineage analysis). However, reconstruction of the developing vulva from EM serial sections has suggested seven different cell types (vulA, vulB1, vulB2, vulC, vulD, vulE, and vulF), many of which cannot be distinguished based on such observations. Here we report the vulval expression of seven genes, egl-17, cdh-3, ceh-2, zmp-1, B0034.1, T04B2.6 and F47B8.6 based on gfp, cfp and yfp (green fluorescent protein and color variants) reporter fusions. Each gene expresses in a specific subset of vulval cells, and is therefore useful as a marker for vulval cell fates. Together, expressions of markers distinguish six cell types, and reveal a strict temporal control of gene expression in the developing vulva. (c) 2003 Elsevier Science Ireland Ltd. All rights reserved.