The see-through medaka:: A fish model that is transparent throughout life
The see-through medaka:: A fish model that is transparent throughout life
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DOI：
10.1073/pnas.181204298
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发表时间：
2001-08-28
影响因子：
11.1
通讯作者：
Ozato, K
中科院分区：
文献类型：
--
作者：
Wakamátsu, Y;Pristyazhnyuk, S;Ozato, K
透明青鳉是一种脊椎动物模型，在成体阶段以及胚胎阶段具有透明的身体，它是由小型实验室鱼青鳉（Oryzias latipes）产生的。在这个鱼模型中，大部分色素通过四个基因座上的隐性等位基因的组合从整个身体中遗传去除。成鱼的主要内脏器官，即心、脾、血管、肝、肠、性腺、肾、脑、脊髓、透镜、气囊和鳃，用肉眼或用简单的立体显微镜都能看到。这条鱼是健康和肥沃的。通过将绿色荧光蛋白（GFP）基因与青鳉vasa基因的调控区融合，产生转基因透明青鳉，其中GFP的生殖细胞特异性表达是可视化的。荧光标签还有效地提高了性腺组织的可见性。卵巢中卵母细胞成熟的过程通过在转基因透明种群的相同活雌性中的一个产卵周期期间从体外重复观察来监测。透明青鳉将为非侵入性研究生命后期内脏器官中发生的形态和分子事件提供机会。
The see-through medaka is a vertebrate model with a transparent body in the adult stage, as well as during the embryonic stages, that was generated from a small laboratory fish, medaka (Oryzias latipes). In this fish model, most of the pigments are genetically removed from the entire body by a combination of recessive alleles at four loci. The main internal organs, namely, heart, spleen, blood vessels, liver, gut, gonads, kidney, brain, spinal cord, lens, air bladder, and gills, in living adult fish are visible to the naked eye or with a simple stereoscopic microscope. This fish is healthy and fertile. A transgenic see-through medaka was produced by using the green fluorescent protein (GFP) gene fused to the regulatory regions of the medaka vasa gene, in which germ cell-specific expression of GFP was visualized. The fluorescent tag also efficiently improved visibility of gonadal tissues. The process of oocyte maturation in the ovary was monitored by repeated observations from the outside of the body during one spawning cycle in the same living females of the transgenic see-through stock. The see-through medaka will provide an opportunity for noninvasive studies of morphological and molecular events that occur in internal organs in the later stages of life.