Effect of synthetic colloids on major histocompatibility complex class II expression
Effect of synthetic colloids on major histocompatibility complex class II expression
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DOI：
10.1016/j.jclinane.2005.06.010
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发表时间：
2006-03-01
影响因子：
6.7
通讯作者：
Dieterich, HJ
中科院分区：
文献类型：
--
作者：
Haeberle, HA;Nohé, B;Dieterich, HJ
研究目的：合成胶体用于围手术期液体管理。我们假设它们的使用可能与主要组织相容性复合体 (MHC) II 类表达的变化有关。这可能会影响临床干预期间患者的发病率和死亡率。地点：大学研究实验室。受试者：健康志愿者的全血样本。干预措施：将健康志愿者 (n = 6) 的全血样本与不同浓度的玉米和马铃薯羟乙基淀粉 (HES) (pHES)、葡聚糖和聚明胶 (明胶) 一起孵育 24 小时，有或没有 100 U/mL 人干扰素 γ (IFN-gamma；刺激MHC II 类表达）。使用异硫氰酸荧光素 (FITC) 标记的抗体同时检测人类白细胞抗原 (HLA)：HLA-DR、HLA-DQ 和 HLA-DP 的表达，并通过流式细胞术对淋巴细胞和单核细胞进行分析。测量和主要结果：羟乙基淀粉、pHES 和葡聚糖诱导 HLA 表达显着增加。 II 类 MHC 的诱导与结构无关（50 mg/mL：对照，8.7 +/- 1.4%；HES，28 +/- 9.7%；pHES，29.8 +/- 11.7%；葡聚糖，50.2 +/- 8.1%）。相比之下，聚明胶仅在最高浓度的明胶（5 mg/mL，7.8 +/- 1%；50 mg/mL，7.6 +/- 0.8%；100 mg/mL，7.3 +/- 1%；200 mg/mL，16.2 +/- 2.3%）下增加HLA表达。在最高浓度的 HES 和右旋糖酐存在下，添加 IFN-γ 会降低 HLA 表达。结论：在离体实验室环境中，我们证明高浓度的血浆扩容剂与淋巴细胞和单核细胞上的 HLA 表达增加有关。然而，需要进一步的体内研究来证明这一观察结果的临床意义。 (c) 2006 Elsevier Inc. 保留所有权利。
Study Objective: Synthetic colloids are used for perioperative fluid management. We hypothesized that their use may be associated with changes in major histocompatibility complex (MHC) class II expression. This could affect patients' morbidity and mortality during clinical intervention.Setting: University research laboratory.Subjects: Whole blood samples from healthy volunteers.Interventions: Whole blood samples from healthy volunteers (n = 6) were incubated with different concentrations of hydroxyethyl starch (HES) from maize and potato (pHES), dextran, and polygelin (gelatine) for 24 hours with or without 100 U/mL human interferon gamma (IFN-gamma; stimulus for MHC class II expression). The expression of human leukocyte antigen (HLA): HLA-DR, HLA-DQ, and HLA-DP was detected simultaneously by a fluoresceinisothiocyanate (FITC)-labeled antibody and analyzed by flow cytometry on lymphocytes and monocytes.Measurements and Main Results: Hydroxyethyl starch, pHES, and dextran induced a significant increase in HLA expression. The induction of MHC class II was independent of the structure (50 mg/mL: control, 8.7 +/- 1.4%; HES, 28 +/- 9.7%; pHES, 29.8 +/- 11.7%; and dextran, 50.2 +/- 8.1%). In contrast, polygelin increased HLA expression only at the highest concentration of gelatine (5 mg/mL, 7.8 +/- 1%; 50 mg/mL, 7.6 +/- 0.8%; 100 mg/mL, 7.3 +/- 1%; 200 mg/mL, 16.2 +/- 2.3%). The addition of IFN-gamma decreased HLA expression in presence of highest concentration of HES and dextran.Conclusion: In an ex vivo laboratory setting, we demonstrate that high concentrations of plasma expanders are associated with increased HLA expression on lymphocytes and monocytes. However, further in vivo studies are necessary to demonstrate the clinical significance of this observation. (c) 2006 Elsevier Inc. All rights reserved.