Plant-in-chip: Microfluidic system for studying root growth and pathogenic interactions in Arabidopsis
Plant-in-chip: Microfluidic system for studying root growth and pathogenic interactions in Arabidopsis
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DOI：
10.1063/1.3604788
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发表时间：
2011-06-27
影响因子：
4
通讯作者：
Pandey, Santosh
中科院分区：
文献类型：
--
作者：
Parashar, Archana;Pandey, Santosh
我们报告了一个用于拟南芥水培生长的微流控平台，该平台具有高分辨率的根系发育和根-病原体相互作用的可视化。该平台包括一组具有单独输入/输出端口的平行微通道，其中最初放置1天大的发芽幼苗。在最佳条件下，根系生长在每个微通道和其图像记录超过198小时的时间。不同浓度的植物生长介质表现出不同的根系生长特性。随后，用两种植物病原体（线虫和游动孢子）接种发达的根，观察它们与活根系的物理化学相互作用。(C)2011年美国物理学会。[doi：10.1063/1.3604788]
We report a microfluidic platform for the hydroponic growth of Arabidopsis plants with high-resolution visualization of root development and root-pathogen interactions. The platform comprises a set of parallel microchannels with individual input/output ports where 1-day old germinated seedlings are initially placed. Under optimum conditions, a root system grows in each microchannel and its images are recorded over a 198-h period. Different concentrations of plant growth media show different root growth characteristics. Later, the developed roots are inoculated with two plant pathogens (nematodes and zoospores) and their physicochemical interactions with the live root systems are observed. (C) 2011 American Institute of Physics. [doi:10.1063/1.3604788]