Effect of breastfeeding on immunogenicity of oral live-attenuated human rotavirus vaccine: a randomized trial in HIV-uninfected infants in Soweto, South Africa
Effect of breastfeeding on immunogenicity of oral live-attenuated human rotavirus vaccine: a randomized trial in HIV-uninfected infants in Soweto, South Africa
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DOI：
10.2471/blt.13.128066
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发表时间：
2014-04-01
影响因子：
11.1
通讯作者：
Madhi, Shabir A.
中科院分区：
文献类型：
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作者：
Groome, Michelle J.;Moon, Sung-Sil;Madhi, Shabir A.
目的探讨预防接种前后母乳喂养1 h对婴儿接种两剂轮状病毒疫苗（RV vaccine.Methods）后免疫应答的影响。方法在南非索韦托，对未感染人类免疫缺陷病毒（HIV）的母婴配对进行为期6周的轮状病毒疫苗接种。每名婴儿被随机分配到第1组，在每剂轮状病毒疫苗之前和之后推迟母乳喂养至少1小时，或第2组，鼓励无限制母乳喂养。酶联免疫吸附试验被用来评估滴度的轮状病毒特异性伊加的血清样本收集每个婴儿立即。每次接种前和第二次接种后1个月。在婴儿中，轮状病毒特异性伊加的滴度增加四倍或更大的疫苗接种后被认为是seroconversion.Findings的指示组1（n = 98）中的可评估的婴儿是相似的，在组2（n = 106）在他们的基线人口统计学特征和他们的疫苗接种前抗轮状病毒伊加滴度。第二次接种疫苗后，第1组婴儿血清中抗轮状病毒伊加的几何平均滴度与第2组婴儿血清中的相似第1组婴儿的血清转换频率与第2组婴儿相似（P=0.685）结论在未感染HIV的南非婴儿中，在每次接种疫苗之前和之后至少1小时停止母乳喂养对婴儿对轮状病毒疫苗
Objective To investigate the effect of abstention from breastfeeding, for an hour before and after each vaccination, on the immune responses of infants to two doses of rotavirus vaccine.Methods In Soweto, South Africa, mother infant pairs who were uninfected with human immunodeficiency virus (HIV) were enrolled as they presented for the "6-week" immunizations of the infants. Each infant was randomly assigned to Group 1 in which breastfeeding was deferred for at least 1 h before and after each dose of rotavirus vaccine or Group 2 in which unrestricted breastfeeding was encouraged. Enzyme-linked immunosorbent assays were used to evaluate the titres of rotavirus-specific IgA in samples of serum collected from each infant immediately. before each vaccine dose and 1 month after the second dose. Among the infants, a fourfold or greater increase in titres of rotavirus-specific IgA following vaccination was considered indicative of seroconversion.Findings The evaluable infants in Group 1 (n = 98) were similar to those in Group 2 (n = 106) in their baseline demographic characteristics and their pre-vaccination titres of anti-rotavirus IgA. After the second vaccine doses, geometric mean titres of anti-rotavirus IgA in the sera of Group-1 infants were similar to those in the sera of Group-2 infants (P=0.685) and the frequency of seroconversion in the Group-1 infants was similar to that in the Group-2 infants (P=0.485).Conclusion Among HIV-uninfected South African infants, abstention from breastfeeding for at least 1 h before and after each vaccination dose had no significant effect on the infants' immune response to a rotavirus vaccine.