COLOCALIZATION OF PEPTIDE AND GLUCOCORTICOID RECEPTOR IMMUNOREACTIVITIES IN RAT CENTRAL AMYGDALOID NUCLEUS
COLOCALIZATION OF PEPTIDE AND GLUCOCORTICOID RECEPTOR IMMUNOREACTIVITIES IN RAT CENTRAL AMYGDALOID NUCLEUS
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DOI：
10.1159/000126156
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发表时间：
1992-04-01
影响因子：
4.1
通讯作者：
HOKFELT, T
中科院分区：
文献类型：
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作者：
HONKANIEMI, J;PELTOHUIKKO, M;HOKFELT, T
杏仁中央核（ACe）是杏仁复合体的一部分，参与促肾上腺皮质激素分泌，应激相关反应和行为功能。 ACe含有大量的糖皮质激素受体（GR）免疫反应（IR）神经元，此外，它已被证明含有几个神经肽IR胞体和神经末梢。 为了研究GR-和神经肽-IR结构之间的关系，我们绘制了GR样免疫反应（LI）在杏仁复合体中的分布，并将神经肽-和GR-LI共定位在ACe中。 在杏仁复合体的中央，内侧和皮质核含有大量的GR-IR神经元，而在基底外侧和基底内侧核中观察到中等数量的GR-IR神经元。 仅在外侧核可见少量GR阳性神经元。 在ACe中，大多数促肾上腺皮质激素释放因子（CRF）、甲硫氨酸脑啡肽（met-ENK）、神经降压素（NT）和生长抑素（SOM）阳性神经元也含有GR-IR，约半数的P物质（SP）阳性神经元含有GR-IR，而仅有少数血管活性肠肽和胆囊收缩素阳性神经元含有GR-IR。 含有降钙素基因相关肽和上述肽的神经末梢与GR-IR神经元紧密接触。 提示糖皮质激素可直接调节ACe中CRF-、met-ENK-、NT-、SOM-和SP-IR细胞的神经递质合成。
The central amygdaloid nucleus (ACe) is part of the amygdaloid complex that participates in adrenocorticotrophin secretion, stress-related reactions and behavioral functions. The ACe contains numerous glucocorticoid receptor (GR)-immunoreactive (IR) neurons, and in addition it has been shown to contain several neuropeptide-IR somata and nerve terminals. In order to study the relationship between the GR- and neuropeptide-IR structures we mapped the distribution of GR-like immunoreactivity (LI) in amygdaloid complex and colocalized the neuropeptide- and GR-LIs in the ACe. In the amygdaloid complex the central, medial and cortical nuclei contained a high number of GR-IR neurons, whereas a moderate number of GR-IR neurons were observed in the basolateral and basomedial nuclei. Only a few GR-IR neurons were seen in the lateral nucleus. In the ACe, the majority of corticotrophin-releasing factor (CRF)-, met-enkephalin (met-ENK)-, neurotensin (NT)- and somatostatin (SOM)-IR neurons contained also GR-IR. About half of the substance P (SP)-IR neurons were seen to contain GR-IR, whereas only some of the few vasoactive intestinal polypeptide and cholecystokinin-IR neurons showed GR-LI. Nerve terminals containing calcitonin gene-related peptide and the above mentioned peptides were seen in close contact with the GR-IR neurons. These results suggest that the glucocorticoids may modulate directly the neurotransmitter synthesis of the CRF-, met-ENK, NT-, SOM- and SP-IR cells in the ACe.