Controlled feeding of lignocellulosic substrate enhances the performance of fed-batch enzymatic hydrolysis in a stirred tank reactor.
Controlled feeding of lignocellulosic substrate enhances the performance of fed-batch enzymatic hydrolysis in a stirred tank reactor.
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木质纤维素底物的受控进料增强了搅拌釜反应器中分批补料酶水解的性能。
DOI：
10.1016/j.biombioe.2016.05.037
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发表时间：
2016
影响因子：
6
通讯作者：
J. Tanskanen
中科院分区：
文献类型：
--
作者：
Ville;Sanna Taskila;H. Ojamo;J. Tanskanen
在高固体（>15% (w/v)）中，木质纤维素酶解的高初始粘度是有问题的，特别是在搅拌槽反应器概念中。避免高粘度的一种潜在方法是将木质纤维素材料分批进料到反应器中。在本研究中，对终浓度为19.1% （w/w）的滤纸，采用不同的进料批次工艺进行了水解评价。加料依据目测、分级加料和搅拌电机功率要求进行。所有进料-间歇工艺在30 h内的产率相似（47-49%），高于类似反应器中间歇工艺的产率（38%）。然而，功率加料的混合性能较好，因为搅拌电机的瞬时功率（<10 W）低于其他加料过程（约20 W）。在不同的酶剂量、尖端速度和底物饲料功率水平下，进一步评估了功率控制过程。进一步的研究表明，如果正确设置底物进料的功率水平，功率控制进料也适用于其他水解和混合条件。较高（15 FPU/g）的酶用量较低（5 FPU/g）的酶用量（7.0±1.3 h和33±5 Wh）缩短了摄食时间（3.0±0.5 h），降低了摄食期间的能量消耗（14±3 Wh）。针尖速度和基板进给功率对这些因素的影响较小。因此，通过搅拌电机的功率控制底物进料，可以提高水解过程的性能。
High initial viscosity in the high-solids (>15% (w/v)) enzymatic hydrolysis of lignocellulose is problematic especially in stirred tank reactor concepts. One potential way to avoid the high viscosity is the fed-batch feeding of lignocellulosic material to the reactor. In the current study the hydrolysis of filter paper with final concentration of 19.1% (w/w) was evaluated with different fed-batch procedures. Feeding was based on visual observation, stepwise feeding and the power requirement of the stirrer motor. All the fed-batch procedures resulted in similar yields within 30 h (47–49%) which were higher than with the batch process in similar reactor (38%). However, the mixing behavior was superior in the power based feeding as the instantaneous power of the stirrer motor was kept lower (<10 W) than in other fed-batch procedures (>20 W). The power controlled procedure was further evaluated with different enzyme doses, tip speeds and the power levels of substrate feed. Further study showed that the power controlled feeding is applicable also to other hydrolysis and mixing conditions if power levels of substrate feed are set correctly. Higher (15 FPU/g) enzyme dose caused shorter feeding time (3.0 ± 0.5 h) and lower energy consumption during the feeding period (14 ± 3 Wh) compared with lower (5 FPU/g) enzyme dose (7.0 ± 1.3 h and 33 ± 5 Wh, respectively). The tip speed and the power level of substrate feed had fewer effect on these factors. The performance of the hydrolysis process can thus be enhanced by the substrate feed controlled by the power of the stirrer motor.