Regulation of continuous but complex expression pattern of Six1 during early sensory development
Regulation of continuous but complex expression pattern of Six1 during early sensory development
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早期感觉发育过程中 Six1 连续但复杂的表达模式的调节
DOI：
10.1002/dvdy.24603
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kawakami K
中科院分区：
文献类型：
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作者：
Sato S;Furuta Y;Kawakami K
背景：在脊椎动物中，颅感觉基板产生神经感觉和内分泌结构，如嗅上皮、内耳和垂体前叶。结果：在新的Cre表达系mSix 1 - 21-NLSCre中，Cre介导的重组在胚胎第8.5天在与耳-上鳃前体结构域（OEPD）重叠的区域被诱导，OEPD是耳和上鳃基板的一个短暂的、共同的前体结构域。随后在OEPD衍生结构（整个内耳和VII-X颅感觉神经节）、嗅上皮、垂体前叶、咽外胚层和囊中观察到肿胀。唾液腺/泪腺和肢芽等61 Six 1阳性结构也为重组阳性。此外，与另一种在感觉神经元增强子Six 1 - 8控制下表达Cre的小鼠品系进行比较，表明在感觉器官形成期间Six 1的连续和复杂表达模式是由单独的增强子拼凑在一起的。结论：mSix 1 - 21-NLSCre具有几个独特的特征，使其适用于分析感觉基板以及非基板Six 1-阳性结构中的细胞谱系和基因功能。Developmental Dynamics 247：250-261，2018。© 2017威利期刊公司.
Background:In vertebrates, cranial sensory placodes give rise to neurosensory and endocrine structures, such as the olfactory epithelium, inner ear, and anterior pituitary. We report here the establishment of a transgenic mouse line that expresses Cre recombinase under the control of Six1‐21, a major placodal enhancer of the homeobox geneSix1.Results:In the new Cre‐expressing line, mSix1‐21‐NLSCre, the earliest Cre‐mediated recombination was induced at embryonic day 8.5 in the region overlapping with the otic‐epibranchial progenitor domain (OEPD), a transient, common precursor domain for the otic and epibranchial placodes. Recombination was later observed in the OEPD‐derived structures (the entire inner ear and the VIIth–Xth cranial sensory ganglia), olfactory epithelium, anterior pituitary, pharyngeal ectoderm and pouches. OtherSix1‐positive structures, such as salivary/lacrimal glands and limb buds, were also positive for recombination. Moreover, comparison with another mouse line expressing Cre under the control of the sensory neuron enhancer, Six1‐8, indicated that the continuous and complex expression pattern ofSix1during sensory organ formation is pieced together by separate enhancers.Conclusions:mSix1‐21‐NLSCre has several unique characteristics to make it suitable for analysis of cell lineage and gene function in sensory placodes as well as nonplacodalSix1‐positive structures.Developmental Dynamics 247:250–261, 2018. © 2017 Wiley Periodicals, Inc.