Signaling from Plastid Genome Stability Modulates Endoreplication and Cell Cycle during Plant Development
Signaling from Plastid Genome Stability Modulates Endoreplication and Cell Cycle during Plant Development
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来自质体基因组稳定性的信号调节植物发育过程中的内复制和细胞周期
DOI：
10.1016/j.celrep.2020.108019
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发表时间：
2020-08-11
期刊：
影响因子：
8.8
通讯作者：
Wang, Hong-Bin
中科院分区：
文献类型：
--
作者：
Duan, Sujuan;Hu, Lili;Wang, Hong-Bin
质体-核基因组协调对质体活性至关重要，但其机制尚不清楚。通过用细胞器基因组损伤剂环丙沙星处理拟南芥植物，我们发现质体基因组的不稳定性可以改变内复制和细胞周期。在reca 1 why 1 why 3的质体基因组不稳定突变体中也观察到类似的结果。在reca 1 why 1 why 3突变体中细胞分裂和胚胎发育受到干扰。值得注意的是，编码细胞周期激酶抑制剂的SMR 5和SMR 7基因在质体基因组不稳定植物中上调，并且SMR 7的突变可以恢复reca 1 why 1 why 3植物的内复制和生长表型。此外，我们建立了DNA损伤反应转录因子SOG 1介导的质体基因组不稳定性引发的内复制和细胞周期的改变。最后，我们证明了质体中产生的活性氧对质体-核基因组协调是重要的。我们的研究结果揭示了植物生长发育过程中质体和核基因组协调的分子机制。
Plastid-nucleus genome coordination is crucial for plastid activity, but the mechanisms remain unclear. By treating Arabidopsis plants with the organellar genome-damaging agent ciprofloxacin, we found that plastid genome instability can alter endoreplication and the cell cycle. Similar results are observed in the plastid genome instability mutants of reca1why1why3. Cell division and embryo development are disturbed in the reca1why1why3 mutant. Notably, SMR5 and SMR7 genes, which encode cell-cycle kinase inhibitors, are upregulated in plastid genome instability plants, and the mutation of SMR7 can restore the endoreplication and growth phenotype of reca1why1why3 plants. Furthermore, we establish that the DNA damage response transcription factor SOG1 mediates the alteration of endoreplication and cell cycle triggered by plastid genome instability. Finally, we demonstrate that reactive oxygen species produced in plastids are important for plastid-nucleus genome coordination. Our findings uncover a molecular mechanism for the coordination of plastid and nuclear genomes during plant growth and development.