The acquisition of thermal tolerance in larvae of calpodes ethlius (lepidoptera) and the in situ and in vitro synthesis of heat-shock proteins.
The acquisition of thermal tolerance in larvae of calpodes ethlius (lepidoptera) and the in situ and in vitro synthesis of heat-shock proteins.
复制标题
Calpodes ethlius（鳞翅目）幼虫热耐受性的获得以及热激蛋白的原位和体外合成。
DOI：
10.1139/o83-063
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
B. Atkinson
中科院分区：
文献类型：
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作者：
R. Dean;B. Atkinson
在22 ℃下饲养的Calpodes ethlius（鳞翅目）幼虫在45 ℃下1小时后死亡，但如果它们首先暴露于37 ℃ 1或2小时，则它们获得在45 ℃下耐受1小时的能力。孵化的完整幼虫在37摄氏度为1小时诱导新的和（或）增强合成的类似家族的多肽（相对质量（MrS）约22 000）在所有组织检查（丝腺，脂肪体，翅盘，中枢神经系统和肌肉）。当这些相同的组织在37摄氏度培养1小时，新的和（或）至少5个多肽（MrS约95 000，81 000，74 000，26 000，和22 000）的合成增强是明显的。培养的组织对热休克的反应更剧烈，推测是由于解剖的额外压力。新的和（或）增强合成的多肽与MrS约22 000的完整的幼虫，在37 ℃孵育1小时的组织，可能与收购的耐热性类似处理的幼虫。这些结果表明，这些多肽的诱导合成是参与耐热性发展的普遍存在的分子机制的一个组成部分。
Larvae of Calpodes ethlius (Lepidoptera) reared at 22 degrees C die after 1 h at 45 degrees C, but they acquire the ability to tolerate 1 h at 45 degrees C if they are first exposed to 37 degrees C for 1 or 2 h. Incubation of intact larvae at 37 degrees C for 1 h induces the new and (or) enhanced synthesis of a similar family of polypeptides (relative masses (MrS) approximately 22 000) in all tissues examined (silk gland, fat body, wing discs, central nervous system, and muscle). When these same tissues are cultured at 37 degrees C for 1 h, the new and (or) enhanced synthesis of at least 5 polypeptides (MrS approximately 95 000, 81 000, 74 000, 26 000, and 22 000) is evident. The more dramatic response elicited from cultured tissues by heat shock results, presumably, from the added stress of dissection. The new and (or) enhanced synthesis of polypeptides with MrS of approximately 22 000 by tissues of intact larvae, incubated at 37 degrees C for 1 h, may be related to the acquisition of thermal tolerance by similarly treated larvae. These results suggest that the induced synthesis of these polypeptides is an integral part of a ubiquitous molecular mechanism involved in the development of thermal tolerance.