Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative.
Production of a Light-Gated Proton Channel by Replacing the Retinal Chromophore with Its Synthetic Vinylene Derivative.
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通过用其合成亚乙烯基衍生物代替视网膜发色团来生产光门控质子通道。
DOI：
10.1021/acs.jpclett.8b00879
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Y. Sudo
中科院分区：
文献类型：
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作者：
R. Takayama;A. Kaneko ;T. Okitsu;S. P. Tsunoda;K. Shimono ;M. Mizuno;K. Kojima;T. Tsukamoto;H. Kandori;Y. Mizutani;A. Wada;Y. Sudo
视紫红质作为一种膜包埋的感光蛋白广泛分布于生物体中，由脱辅基蛋白视蛋白和维生素A醛视网膜组成，A1-视网膜和A2-视网膜是天然发色团。视蛋白的修饰（例如，通过突变）提供了对视紫红质的光诱导功能的分子机制的洞察，以及提供了化学生物学中通过光控制细胞活性的工具。在这项研究中，我们的重点是视网膜的发色团，而不是载脂蛋白视蛋白，合成三个亚乙烯基衍生物的A2-视网膜。其中一个分子C（14）-vinylene A2-retinal（14 V-A2）被成功地整合到光驱动质子泵archaerhodopsin-3（AR 3）的视蛋白中。电生理实验表明，AR 3的视蛋白（archaeopsin 3，AO 3）与14 V-A2作为一个光门控质子通道的功能。工程化的质子通道显示出特征性的光化学性质，这与AR 3的那些显著不同。因此，我们成功地通过替换AR 3的发色团产生了质子通道。
Rhodopsin is widely distributed in organisms as a membrane-embedded photoreceptor protein, consisting of the apoprotein opsin and vitamin-A aldehyde retinal, A1-retinal and A2-retinal being the natural chromophores. Modifications of opsin (e.g., by mutations) have provided insight into the molecular mechanism of the light-induced functions of rhodopsins as well as providing tools in chemical biology to control cellular activity by light. Instead of the apoprotein opsin, in this study, we focused on the retinal chromophore and synthesized three vinylene derivatives of A2-retinal. One of them, C(14)-vinylene A2-retinal (14V-A2), was successfully incorporated into the opsin of a light-driven proton pump archaerhodopsin-3 (AR3). Electrophysiological experiments revealed that the opsin of AR3 (archaeopsin3, AO3) with 14V-A2 functions as a light-gated proton channel. The engineered proton channel showed characteristic photochemical properties, which are significantly different from those of AR3. Thus, we successfully produced a proton channel by replacing the chromophore of AR3.