The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra.
The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra.
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DOI：
10.1016/j.gdata.2014.10.021
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Chen L
中科院分区：
文献类型：
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作者：
Ghani MA;Li J;Rao L;Raza MA;Cao L;Yu N;Zou X;Chen L
小分子RNA在维持植物基因组的重建和稳定中起着重要的作用。然而，小RNA在广泛杂交和染色体加倍过程中的作用知之甚少。因此，本研究对芜菁（Brassica rapa）和黑芥（Brassica nigra）异源二倍体（基因组：AB）及其异源四倍体（基因组：AABB）形成过程中小分子RNA的变化进行了分析。在此，详细描述了实验方法、RNA-seq数据（可在Gene Expression Omnibus数据库中获得，编号为GSE 61872）和Ghani等人发表的分析[1]。研究表明，小分子RNA在维持基因组稳定性，调节基因表达，诱导异源四倍体形成的表型变异中起重要作用。这可能对异源四倍体杂种优势的产生起重要作用。
Small RNAs play an important role in maintaining the genome reconstruction and stability in the plant. However, little is known regarding the role of small RNAs during the process of wide hybridisation and chromosome doubling. Therefore, the changes in the small RNAs were assessed during the formation of an allodiploid (genome: AB) and its allotetraploid (genome: AABB) between Brassica rapa (♀) and Brassica nigra (♂) in the present study. Here, the experimental methods described in details, RNA-seq data (available at Gene Expression Omnibus database under GSE61872) and analysis published by Ghani et al. [1]. The study showed that small RNAs play an important role in maintaining the genome stability, and regulate gene expression which induces the phenotype variation in the formation of an allotetraploid. This may play an important role in the occurrence of heterosis in the allotetraploid.