BIOSYNTHESIS OF THE TARGET ANTIGENS OF ANTIBODIES BLOCKING TRANSMISSION OF PLASMODIUM-FALCIPARUM
BIOSYNTHESIS OF THE TARGET ANTIGENS OF ANTIBODIES BLOCKING TRANSMISSION OF PLASMODIUM-FALCIPARUM
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DOI：
10.1016/0166-6851(84)90124-5
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发表时间：
1984-01-01
影响因子：
1.5
通讯作者：
CARTER, R
中科院分区：
文献类型：
--
作者：
KUMAR, N;CARTER, R
研究了先前在恶性疟原虫的细胞外配子表面上鉴定的Mr [分子比]为260，000、59，000和53，000的3种蛋白质的生物合成，这些蛋白质是阻断恶性疟原虫对蚊子的感染性的单克隆抗体的靶标。在用[35S]甲硫氨酸标记的恶性疟原虫脉冲培养物中，发现这些蛋白质由配子体从其成熟的早期阶段合成，但不由无性血液阶段寄生虫合成。由配子母细胞合成的靶蛋白在细胞外配子的表面上表达，但配子本身不再合成这些蛋白。59，000和53，000 Mr蛋白质不是由260，000 Mr蛋白质加工产生的。59，000和53，000 Mr蛋白质，但不是260，000 Mr蛋白质，被葡糖胺或甘露糖糖基化。
The biosynthesis of 3 proteins of Mr [molecular ratio] 260,000, 59,000 and 53,000 previously identified on the surface of extracellular gametes of P. falciparum as the targets of monoclonal antibodies which block infectivity of P. falciparum to mosquitoes were studied. In cultures of P. falciparum pulse labeled with [35S]methionine it was found that these proteins are synthesized by gametocytes from an early stage in their maturation but are not synthesized by asexual blood stage parasites. The target proteins synthesized by the gametocytes become expressed on the surface of the extracellular gametes but the gametes themselves no longer synthesize these proteins. The 59,000 and 53,000 Mr proteins do not result from processing from the 260,000 Mr protein. The 59,000 and 53,000 Mr protein, but not the 260,000 Mr protein, were glycosylated by either glucosamine or mannose.