Characterization of the eosinophil chemokine RANTES in nasal polyps
Characterization of the eosinophil chemokine RANTES in nasal polyps
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DOI：
10.1177/000348949810700510
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发表时间：
1998-05-01
影响因子：
1.4
通讯作者：
Kreutzer, D
中科院分区：
文献类型：
--
作者：
Allen, JS;Eisma, R;Kreutzer, D
先前的研究已经证明，细胞因子RANTES（调节和正常T细胞表达和分泌）已被证明是体外嗜酸性粒细胞趋化性和白细胞募集的有效介质。由于嗜酸性粒细胞是鼻息肉病的标志细胞，我们假设RANTES是在鼻息肉微环境中局部产生的，并负责鼻息肉病中观察到的嗜酸性粒细胞募集。为了开始验证这一假设，我们使用免疫组织化学技术和酶联免疫吸附测定技术评估了17例鼻息肉患者和3例对照标本中RANTES的分布和含量。我们的免疫组化研究表明，在鼻息肉病，RANTES抗原染色主要发生在嗜酸性粒细胞和上皮细胞。为了定量正常和鼻息肉标本中RANTES的相对水平，制备组织匀浆，定量，并标准化为蛋白质水平。我们在所有17个鼻息肉组织匀浆中检测到RANTES（566 +/- 16 pg/mg总蛋白）。鼻息肉匀浆中的RANTES水平比正常组织中的RANTES水平高近40倍（15.7 ± 28.2 pg/mg总蛋白）。因此，鼻息肉微环境中嗜酸性粒细胞和上皮细胞的RANTES表达增加似乎促进了鼻息肉内嗜酸性粒细胞的募集和激活。我们假设RANTES诱导嗜酸性粒细胞的募集和活化增加，可能导致鼻息肉病相关组织变化增加。
Previous studies have demonstrated that the cytokine RANTES (Regulated And Normal T cell Expressed and Secreted) has been shown to be a potent mediator of eosinophil chemotaxis in vitro and of leukocyte recruitment. Because eosinophils are the hallmark cells in nasal polyposis, we hypothesize that RANTES is locally produced within the nasal polyp microenvironment and is responsible for the eosinophil recruitment seen in nasal polyposis. To begin to test this hypothesis, we evaluated nasal polyps from 17 patients and 3 control specimens for distribution and content of RANTES using immunohistochemical techniques and enzyme-linked immunosorbent assay technology. Our immunohistochemical studies demonstrated that in nasal polyposis, RANTES antigen staining occurred predominantly within eosinophils and epithelial cells. To quantify the relative levels of RANTES in normal and nasal polyp specimens, tissue homogenates were prepared, quantified, and normalized to protein levels. We detected RANTES in all 17 nasal polyp tissue homogenates (566 +/- 16 pg/mg total protein). The RANTES levels in nasal polyp homogenates were nearly 40-fold higher than the RANTES levels in normal tissue (15.7 +/- 28.2 pg/mg total protein). Thus, it appears that increased expression of RANTES by eosinophils and epithelial cells within the nasal polyp microenvironment promotes eosinophil recruitment and activation within nasal polyps. We hypothesize that RANTES induces increased recruitment and activation of eosinophils, presumably contributing to the increased tissue changes associated with nasal polyposis.