Detection of anti-C1q antibodies and anti-C1q globular head domain antibodies in sera from Chinese patients with lupus nephritis
Detection of anti-C1q antibodies and anti-C1q globular head domain antibodies in sera from Chinese patients with lupus nephritis
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DOI：
10.1016/j.molimm.2009.04.030
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发表时间：
2009-07-01
影响因子：
3.6
通讯作者：
Zhao, Ming-hui
中科院分区：
文献类型：
--
作者：
Tan, Ying;Zhou, Wei;Zhao, Ming-hui
血清抗C1 q抗体与狼疮性肾炎患者的疾病活动相关最近的研究表明，抗C1 q抗体识别C1 q分子的单个A（ghA）、B（ghB）和C（ghC）链的胶原区域和球状头部区域（gC 1 q）。然而，抗gC 1 q抗体的临床意义尚不清楚。为探讨狼疮性肾炎（LN）患者血清中抗C1 q分子不同部位抗体的频率及其临床意义，收集83例经肾活检证实的狼疮性肾炎（LN）患者，于肾活检当天进行血清学检测。以纯化的人C1 q、C1 qGR和重组ghA、ghB、ghC为固相抗原，用ELISA法检测血清中抗C1 q抗体、抗胶原样区（C1 qGR）抗体、抗球状头部区（C1 qGR）抗体和抗ghA、ghB、ghC抗体。抗C1 q和抗C1 qR 2抗体在狼疮肾炎组的阳性率（分别为46/83，55.4%和36/83，43.4%）显著高于非狼疮性系统性红斑狼疮组（4/30，13.3%，P < 0.01）。
Serum anti-C1q antibodies are associated with disease activity in patients with lupus nephritis. Recent studies showed that anti-C1q antibodies recognize both the collagen region and the globular head regions (gC1q) of individual A (ghA), B (ghB), and C (ghC) chains of C1q molecules. However, the clinical significance of anti-gC1q antibodies was not clear. This study is to investigate the frequency of antibodies against different parts of C1q molecule in sera from patients with lupus nephritis and their clinical significance.Sera from 83 patients with renal biopsy-proven lupus nephritis were collected at the day of renal biopsy. Sera from 30 patients with non-renal SLE (NR-SLE) and 100 healthy donors were used as controls, Serum anti-C1q antibodies, anti-collagen-like region (C1qCLR) antibodies, anti-globular head region (C1qGR) antibodies and anti-ghA, anti-ghB and anti-ghC antibodies were screened by ELISA using purified human C1q, C1qCLR, C1qGR and recombinant ghA, ghB and ghC as solid phase antigens. The association of these antibodies and clinical and histopathological features was further studied.The frequencies of positive anti-C1q and anti-C1qCLR antibodies in lupus nephritis group (46/83, 55.4%; 36/83, 43.4%, respectively) were significantly higher than that in patients with NR-SLE (4/30, 13.3%, P