Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa
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DOI：
10.1016/j.devcel.2004.12.005
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发表时间：
2005-03-01
影响因子：
11.8
通讯作者：
Kinoshita, M
中科院分区：
文献类型：
--
作者：
Ihara, M;Kinoshita, A;Kinoshita, M
Septins是胞质分裂和其他细胞过程中皮质组织所需的GTP结合蛋白的聚合体。哺乳动物Septin基因Sept4主要在有丝分裂后神经细胞和减数分裂后雄性生殖细胞中表达。在小鼠和人类的精子中，SEPT4和其他隔质存在于环中，环是分隔中间部分和主体部分的皮质环。9月4日(-/-)雄性小鼠由于精子鞭毛的形态和活力缺陷而不育。在9月4日的零精子中，环被缺乏皮质物质的脆弱部分所取代，在该部分之下，动蛋白介导的鞭毛内运输停滞不前。通过将精子注射到卵母细胞中可以挽救这种不育症，这表明每个9月4日没有精子的精子都携带着一个完整的单倍体基因组。弱精子症患者的精子中的环/隔质环也是杂乱无章的。因此，基于间隔蛋白细胞骨架环状组装的皮质组织对于哺乳动物精子的结构和机械完整性是必不可少的。
Septins are polymerizing GTP binding proteins required for cortical organization during cytokinesis and other cellular processes. A mammalian septin gene Sept4 is expressed mainly in postmitotic neural cells and postmeiotic male germ cells. In mouse and human spermatozoa, SEPT4 and other septins are found in the annulus, a cortical ring which separates the middle and principal pieces. Sept4(-/-) male mice are sterile due to defective morphology and motility of the sperm flagellum. In Sept4 null spermatozoa, the annulus is replaced by a fragile segment lacking cortical material, beneath which kinesin-mediated intraflagellar transport stalls. The sterility is rescued by injection of sperm into oocytes, demonstrating that each Sept4 null spermatozoon carries an intact haploid genome. The annulus/septin ring is also disorganized in spermatozoa from a subset of human patients with asthenospermia syndrome. Thus, cortical organization based on circular assembly of the septin cytoskeleton is essential for the structural and mechanical integrity of mammalian spermatozoa.