Light-Induced Artemisinin Biosynthesis Is Regulated by the bZIP Transcription Factor AaHY5 in Artemisia annual
Light-Induced Artemisinin Biosynthesis Is Regulated by the bZIP Transcription Factor AaHY5 in Artemisia annual
复制标题
青蒿中光诱导的青蒿素生物合成受 bZIP 转录因子 AaHY5 调控
DOI：
10.1093/pcp/pcz084
复制
发表时间：
2019-08-01
影响因子：
4.9
通讯作者：
Tang, Kexuan
中科院分区：
文献类型：
--
作者：
Hao, Xiaolong;Zhong, Yijun;Tang, Kexuan
青蒿素是从黄花蒿中提取的一种倍半萜类化合物，是治疗疟疾的一线药物。已经提出光在青蒿素生物合成的激活中起重要作用。在这里，我们报告了碱性亮氨酸拉链转录因子（TF）AaHY5作为光诱导青蒿素生物合成的关键调节因子。我们发现，AaHY5的转录与青蒿素生物合成基因的转录重叠，在响应光和A。annua纸巾对AaHY5过表达和RNAi抑制株系的分析表明，AaHY5是青蒿素生物合成基因表达和青蒿素积累的正调控因子。我们表明，AaHY5补充拟南芥中的hy5突变体。我们的数据进一步表明，AaHY5与AaCOP 1相互作用，AaCOP 1是A. annua。在酵母单杂交和瞬时表达试验中，我们证明了AaHY5通过TF腺体特异性WRKY 1（AaGSW 1）在青蒿素调节中起作用。总之，我们提出了一种新的青蒿素基因表达调节剂，并提出了一个模型，其中AaHY5间接控制青蒿素的生产，以应对不断变化的光照条件。
Artemisinin, the frontline drug against malaria, is a sesquiterpenoid extracted from Artemisia annua. Light has been proposed to play an important role in the activation of artemisinin biosynthesis. Here, we report the basic leucine zipper transcription factor (TF) AaHY5 as a key regulator of light-induced biosynthesis of artemisinin. We show that AaHY5 transcription overlaps with that of artemisinin biosynthesis genes in response to light and in A. annua tissues. Analysis of AaHY5 overexpression and RNAi-suppression lines suggests that AaHY5 is a positive regulator of the expression of artemisinin biosynthesis genes and accumulation of artemisinin. We show that AaHY5 complements the hy5 mutant in Arabidopsis thaliana. Our data further suggest that AaHY5 interacts with AaCOP1, the ubiquitin E3 ligase CONSTITUTIVE PHOTOMORPHOGENIC1 in A. annua. In yeast one-hybrid and transient expression assays, we demonstrate that AaHY5 acts via the TF GLANDULAR TRICHOME-SPECIFIC WRKY 1 (AaGSW1) in artemisinin regulation. In summary, we present a novel regulator of artemisinin gene expression and propose a model in which AaHY5 indirectly controls artemisinin production in response to changing light conditions.