Liposome adhesion on mica surface studied by atomic force microscopy
Liposome adhesion on mica surface studied by atomic force microscopy
复制标题
DOI：
10.1021/la980923w
复制
发表时间：
1999-03-02
期刊：
影响因子：
3.9
通讯作者：
Furusawa, K
中科院分区：
文献类型：
--
作者：
Egawa, H;Furusawa, K
用原子力显微镜（AFM）原位记录了磷脂酰胆碱（PC）和磷脂酰乙醇胺（PE）脂质体通过直接粘附在云母表面从囊泡形态到平坦双层的动态形貌变化。对照实验表明，脂质体立即粘附在云母表面上，并且囊泡的球形形状自发地破裂并变形为云母上的平坦支撑的双层。然而，在PC脂质体中，几乎没有形成第二双层，只有饱和的第一双层在整个云母表面上延伸。PC双层的形成速率取决于介质中的盐和囊泡浓度，并随着这些材料的含量增加而增加。而在PE脂质体体系中，脂质分子的垂直生长与PC和PE体系有明显的区别，这种不同的行为可以用PC表面水化层的存在来解释。
The dynamic topography change of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) liposomes from a vesicle form to a flat bilayer through their direct adhesion on a mica surface has been recorded by atomic force microscopy (AFM) in situ. Controlled experiments demonstrated that immediately the liposome adhered on the mica surface and the spherical shape of the vesicle ruptured spontaneously and deformed to a flat supported bilayer on the mica. In the PC Liposome, however, the second bilayer was hardly formed and only a saturated first bilayer was extended over all the mica surface. The formation rate of the PC bilayer depended on the salt and vesicle concentrations in the medium and increased with increasing content of these materials. On the other hand, in the PE liposome system, a vertical growth of lipid molecules was recognized and a clear difference between PC and PE systems has been found. This different behavior has been interpreted by the existence of a hydration layer around the PC surface.