Longitudinal effects of estrogen on mandibular growth and changes in cartilage during the growth period in rats
Longitudinal effects of estrogen on mandibular growth and changes in cartilage during the growth period in rats
复制标题
DOI：
10.1016/j.ydbio.2022.10.007
复制
发表时间：
2022-10-21
影响因子：
2.7
通讯作者：
Tanimoto，Kotaro
中科院分区：
文献类型：
--
作者：
Kubo，Naoki;Awada，Tetsuya;Tanimoto，Kotaro
雌激素是一种类固醇激素，可诱导骨骼生长，并在生长期影响长管骨生长板的软骨内成骨。然而，雌激素对下颌骨髁突软骨内骨化的影响尚不清楚。本研究以去卵巢的Wistar/ST大鼠为模型，研究雌激素对下颌骨生长发育的纵向影响。这些大鼠被给予不同剂量的雌激素。采用纵向CT扫描、组织学染色和酶联免疫吸附试验检测血浆生长激素水平，观察雌激素对下颌骨生长发育的影响。结果表明，在整个生长期内，雌激素以剂量依赖的方式抑制下颌骨的生长。此外，长期给予大鼠高剂量雌激素可导致生长激素在整个生长期内显著增加，增殖层细胞核明显环化，软骨下骨中软骨基质染色加深，下颌骨中雌激素受体(ER)α和β的表达显著抑制。然而，无论雌激素浓度如何，在下颌软骨的后部，ER的表达扩展到肥厚层和增生层。这些结果表明，雌激素在整个生长期都抑制了下颌骨的生长。此外，它还通过对内质网的作用影响软骨内成骨。
Estrogen is a steroid hormone that induces skeletal growth and affects endochondral ossification of the long tubular bone growth plate during the growth period. However, the effects of estrogen on endochondral ossification of the mandibular condylar cartilage are unclear. In this study, ovariectomized Wistar/ST rats were used to investigate the longitudinal effects of estrogen on mandibular growth. The rats were administered different doses of estrogen. Longitudinal micro-computed tomographic scanning, histological staining and ELISA on plasma growth hormone were performed to examine the effects of estrogen on mandibular growth. The results showed that mandibular growth was suppressed throughout the growth period by estrogen in a dose-dependent manner. In addition, long-term administration of a high dose of estrogen to the rats resulted in significant increase in growth hormone throughout the growth period, significant circularization of cell nuclei in the proliferative layer, intensely staining cartilage matrix in the subchondral bone, and significant suppression of estrogen receptor (ER) alpha and beta expression in the mandibular cartilage. However, regardless of estrogen concentration, in the posterior part of the mandibular cartilage, ER expression extended to both the hypertrophic and proliferative layers. These results indicate that estrogen suppresses mandibular growth throughout the growth period. Additionally, it influences endochondral ossification via its effect on ERs.