Approaches to Monitor Termination of DNA Replication Using Xenopus Egg Extracts.
Approaches to Monitor Termination of DNA Replication Using Xenopus Egg Extracts.
复制标题
使用非洲爪蟾卵提取物监测 DNA 复制终止的方法。
DOI：
10.1007/978-1-0716-2063-2_7
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Dewar,JamesM
中科院分区：
文献类型：
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作者：
Kavlashvili,Tamar;Dewar,JamesM
DNA复制对细胞活力和基因组完整性至关重要。尽管它在基因组复制中起着至关重要的作用，但DNA复制的最后阶段，即被称为终止，却相对未被探索。我们对终止的了解受限于研究DNA复制的细胞方法，这种方法不能轻易检测到终止。相比之下，非洲爪蟾卵细胞提取系统可以很容易地检测到所有的DNA复制。在这里，我们描述了使用该系统和分析来监控复制终止。
DNA replication is crucial for cell viability and genome integrity. Despite its crucial role in genome duplication, the final stage of DNA replication, which is termed termination, is relatively unexplored. Our knowledge of termination is limited by cellular approaches to study DNA replication, which cannot readily detect termination. In contrast, theXenopus laevisegg extract system allows for all of DNA replication to be readily detected. Here we describe the use of this system and assays to monitor replication termination.